摘要
目的探讨在小鼠结肠癌肝转移发生之前,门静脉中的肿瘤可溶性产物IL-10、TGF-β1水平与肝脏Th1/Th2细胞因子的变化情况及其相关性。方法 20只BALB/c小鼠随机分为两组:盲肠肿瘤块(CT26细胞)种植法建立原位结肠癌肝转移模型为试验组,对照组为假手术组。2周后提取门静脉血及肝脏标本,ELISA方法检测门静脉血IL-10、TGF-β1及肝脏组织IFN-γ、IL-10浓度,比较两组间差异并计算相关性。结果实验组小鼠肝脏Th1/Th2(IFN-γ/IL-10)比值较对照组降低,且与门静脉IL-10和TGF-β1的浓度呈显著负相关。结论小鼠结肠肿瘤可释放IL-10、TGF-β1入门静脉,导致肝脏Th1/Th2样细胞因子(IFN-γ/IL-10)出现漂移现象,可能会促进肝转移的发生。
Objective To explore the correlation between the level of IL-10 and TGF -β1 in portal vein and the ratio of Th1/Th2 like cytokines in liver previous to liver metastasis in mice models of metastasis. Methods Twenty mice were randomly assigned into control group and experimental group. Established the orthotopic model of liver metastasis by implanting tumor block into the cecal wall in BALB/c mice as experimental group, and the other ten were accepted the sham operation. After two weeks, all mice were killed and blood samples in portal veins and liver samples were collected for ELISA testing of IL-10, TGF-β1 and IFN-y. The t-test was used to compare the differences between two groups, and Bivariate correlation analysis was used to analyze the correlation. Results Compared to the control group,the ratio of Th1/Th2（IFN-y /IL -10） in livers of experimental mice was decreased significantly, which showed a significantly negative correlation with serum levels of IL-10 and TGF-β1 in portal vein. Conclusion Tumors of mouse colon can produce and release IL-10 and TGF β1 into portal vein,which can flow to liver and lead to the shift of Th1/Th2 like cytokines to promote the liver metastasis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第10期1166-1169,共4页
Cancer Research on Prevention and Treatment
基金
广东省科技计划项目基金资助项目(2008B030301128)
