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锌指蛋白(ZNF)185在小鼠不同发育阶段睾丸中的表达 被引量：4

Expression of ZNF185 in Different Developmental Stages of Mouse Testis
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摘要 目的:研究锌指蛋白(ZNF)185在小鼠不同发育阶段睾丸中的表达。方法:从小鼠脑、肝、卵巢和不同发育阶段睾丸组织中提取总RNA,采用半定量RT-PCR法检测ZNF185的表达;制备小鼠不同发育阶段睾丸冰冻切片,应用免疫细胞化学分析法检测ZNF185蛋白的定位。结果:①ZNF185在睾丸中的表达显著高于脑、肝和卵巢组织。②ZNF185在小鼠性成熟前组睾丸中的表达显著低于性成熟期和老年期,而性成熟期和老年期之间差异不显著。③在性成熟前,ZNF185主要定位于睾丸间质细胞和类肌细胞;在性成熟期和老年期小鼠中,ZNF185主要定位于睾丸间质细胞、类肌细胞和精子。结论:ZNF185在小鼠性成熟后睾丸中的表达升高,定位更广泛。 Objective： To study the expression of ZNF185 in different developmental stages of mouse testis. Methods： Total RNA was extracted from mouse brain, liver, ovarian, and testicular tissue in different developmental stages, the expression of ZNF185 was detected by using semi-quantitative RT-PCR assay; the frozen sections of different developmental stages of mouse testis were made, the positioning of ZNF185 was detected using the immunocytochemical analysis. Results： 1） The expression of ZNF 185 in the testis was significantly higher than that in the brain, liver and ovarian tissues. 2） The expression of ZNF 185 in the testes of sexually mature before was significantly lower than that in the sexually mature group and age group, and there was no significant difference between sexually mature group and age group. 3） In the sexually mature before group, ZNF185 mainly located in the leydig cells and myoid cells. In sexually mature group and age group, the ZNF185 mainly located in the Leydig ceils, myoid cells and sperm. Conclusion： The expression of ZNF185 is increased in mouse testis after sexually mature, while the positioning is more widely.
作者 冯卫国 潘智芳 连波 陈丽梅 刘顺梅 刘晓影 高志芹
机构地区 潍坊医学院
出处 《生殖与避孕》 CAS CSCD 2012年第10期672-675,共4页 Reproduction and Contraception
基金 山东省自然科学基金资助项目(ZR2009DL013 ZR2009CL047)
关键词 睾丸 精子发生 锌指蛋白(ZNF)185 RT-PCR 免疫细胞化学 小鼠 testis spermatogenesis ZNF185 RT-PCR immunocytochemistry mice
分类号 R339.21 [医药卫生—人体生理学]
  • 相关文献

参考文献10

  • 1He Z, Kokkinaki M, Dym M. Signaling molecules and pathways the fate of spermatogonial stem cells. Microse Res Tech, 2009, 72(8):586-95.
  • 2Heiss NS, Gloeckner G, Bacher D, et al. Genomic structure of a novel LIM domain gene (ZNF185) in Xq28 and com- parisons with the orthologous murine transcript. Genomics, 1997, 43(3):329-38.
  • 3Rabbitts TH, Boehm T. LIM domains. Nature, 1990, 346 (6283):418.
  • 4Sanchez-Garcia I, Rabbitts TH. The LIM domains: a new structural motif found in zinc-finger-like protein. Trends Genet, 1994, 10(9):315-20.
  • 5Wu X, Blanck A, Norstedt G, et al. Identification of genes with higher expression in human uterine leiomyomas than in the corresponding myometrium. Mol Hum Reprod, 2002, 8(3): 246-54.
  • 6Gonzalez H, Gujrati M, Frederick M, et al. Identification of 9 genes differentially expressed in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg, 2003, 129 (7):754-9.
  • 7Zhang JS, Gong A, Yong CY, et al. ZNF185, an action- cytoskeleton-associated growth inhibitory LIM protein in prostate cancer. Oncogene, 2006, 53(1):2471-6.
  • 8Wang N, Zheng Q, Zhang JS, et al. Molecular cloning and characterization of a novel mouse actin-binding protein Zfp185. J Mol Histol, 2008, 39(3):295-302.
  • 9潘智芳,冯卫国,陈丽梅,刘晓影,赵春玲,连波,于文静,高志芹.ZNF185基因的克隆及在小鼠睾丸中的定位[J].细胞与分子免疫学杂志,2010,26(10):973-975. 被引量：6
  • 10马洁桦,安丽梅,潘连军,黄宇烽.ERβ基因敲除对小鼠阴茎海绵体eNOS-NO通路基因表达的影响[J].生殖与避孕,2012,32(3):145-150. 被引量：2

二级参考文献29

  • 1杨滨,周玉春,潘连军,夏欣一,史轶超,许晓风,黄宇烽.植物雌激素大豆黄酮对大鼠阴茎组织一氧化氮合酶表达的影响[J].生殖与避孕,2009,29(1):7-11. 被引量：6
  • 2蒋春霞,潘连军,黄宇烽.雌激素受体在雄性生殖系统中的分布及其对雄性生殖的作用[J].中华男科学杂志,2007,13(6):550-553. 被引量：10
  • 3Heiss NS, Gloeckner G, Bacher D, et al. Genomic structure of a novel LIM domain gene( ZNF185 )in Xq28 and comparisons with the orthologous murine transcript [ J]. Genomics, 1997, 43 ( 3 ) : 329 - 338.
  • 4Rabbitts TH, Boehm T. LIM domains[J]. Nature, 1990, 346 (6283) : 418.
  • 5Sanchez-Garcia I, Rabbitts T. The LIM domains : a new structural motif found in zinc-finger-like protein[J]. Trends Genet, 1994, 10(9): 315 -320.
  • 6Wu X, Blanck A, Norstedt G, et al. Identification of genes with higher expression in human uterine leiomyomas than in the corresponding myometrium[ J]. Mol Hum Reprod, 2002, 8(3) : 246 -254.
  • 7Gonzalez H, Gujrati M, Frederick M, et al. Identification of 9 genes differentially expressed in head and neck squamous cell carcinoma [ J ]. Arch Otolaryngol Head Neck Surg, 2003, 129 (7) : 754 -759.
  • 8Vanaja D, Cheville J, Iturria S, et al. Transcriptional silencing of zinc finger protein 185 identified by expression profiling is associated wieh prostate cancer progression [ J ]. Cancer Res, 2003, 63 ( 14 ) : 3877 - 3882.
  • 9Wong N, Chan K, Lai P, et al. Transcriptional profiling identifies gene expression changes assoeiated with IFN-alpha tolerance in hepatitis C-related hepatoeellular eareinoma cells [ J]. Clin Cancer Res, 2005, 11(3): 1319-1326.
  • 10Zhang JS, Gong A, Yong CY, et al. ZNF185, an action-cytoskeleton- associated growth inhibitory LIM protein in prostate cancer[ J]. Oncogene, 2006, 53(1): 2471 -2476.

共引文献6

同被引文献43

  • 1邵冰玉,杨占清,吴建英,等.小鼠睾丸间质细胞的分离培养[J].畜牧兽医科学,2008,24(5):26-28.
  • 2Heiss NS, Gloeckner G, Bacher D, et al. Genomic structure of a novel LIM domain gene( ZNF185 ) in Xq28 and comparisons with the orthologous murine transcript [ J ]. Geuomics, 1997,43 ( 3 ) : 329 - 338.
  • 3He Z, Kokkinaki M, Dym M. Signaling molecules and pathways regulating the fate of spermatogonial stem cells [ J ]. Microsc Res Tech,2009,72 (8) :586 - 595.
  • 4Alexandrova ML, Bochev PG. Reduced extracellular phagocyte ox- idative activity, antioxidant level changes and increased oxidative damage in healthy human blood as a function of age [ J ]. Age (Dordr) ,2009,31 (2) :99 - 107.
  • 5EI-Alfy M,Luu-The V,Huang XF,et al. Localization of type 517beta- hydroxysteroid dehydrogenase, 3beta-hydroxysteroiddehydrogenase, and androgen receptor in the human prostate by in situ hybridization and immunoeytochemistry [ J ]. Endocrinology, 1999, 140 (3) : 1481 - 1491.
  • 6Li D, Sekhon P, Barr K J, et al. Connexins and steroidogenesis in mouse Leydig cells [ J ]. Can J Physiol Pharmacol,2013,91 ( 2 ) : 157 - 164.
  • 7Matzkin ME, Yamashita S, Ascoli M. The ERK1/2 pathway regu- lates testosterone synthesis by coordinately regulating the expres- sion of steroidogenic genes in Leydig cells[ J]. Mol Cell Endocri- nol,2013,370( 1 -2) :130 - 137.
  • 8Wang N ,Zheng Q ,Zhang JS ,et al. Molecular cloning and charac- terization of a novel mouse actin-binding protein Zfp185 [ J]. J Mol Histo1,2008,39 ( 3 ) :295 - 302.
  • 9Medina PP, Carretero J, Ballestar E, et al. Transcriptional targets of the chromatin-remodelling factor SMARCA4/BRG1 in lung cancer cells[ J]. Hum Mol Genet,2005,14:973 - 982.
  • 10Zhang JS, Gong A, Young CYF. ZNF185, an actin-cytoskeleton- associated growth inhibitory LIM protein in prostate cancer [J].Oncogene, 2007,26(1):111-122.

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生殖与避孕
2012年 第10期

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