疑难配血中混合抗体鉴定1例

Identification of Mixed Antibodies in a Sample with Doubtful Cross-matching Result

目的探讨疑难配血过程中混合抗体的鉴定方法。方法首先鉴定红细胞血型,并对血清进行抗体筛查及鉴定,再利用特定细胞对患者血清进行吸收放散实验分离抗体,最后分别对分离后的抗体进行鉴定。结果患者血型为O型,Jka+b-,CCDee;用O型CCDee,Jkb+的细胞与患者血清进行吸收放散,分离抗体后鉴定结果为抗-cE、抗-Jkb的混合抗体。结论选择合适的细胞进行吸收放散实验分离抗体,可以实现对混合抗体的鉴定,为患者选择相应抗原阴性的血液,保证临床输血安全。

Objective To identificate the mixed antibodies in a difficult-cross-matching sample. Methods RBC blood groups were identified, serum antibodies were screened and identified, special RBC was used in absorption and elu- tion test,the absorbed serum and the elution liquid were identified separately. Results The patient% blood groups were O, Jka＋b- , CCDee. The RBC with O, CCDee, Jkb＋was used in the absorption and elution test, the mixed antibodies were identified as anti-cE and anti-Jkb. Conclusion Suitable cells used in absorption and elution test can help to identify mixed antibodies. To ensure the safety of blood transfusion, irregular antibody related antigen negative blood can be used for the patient.