电子载体对丁醇发酵的影响

Effects of electronic carrier on butanol fermentation by Clostridium acetobutylicum

研究在培养基中加入不同电子载体对丁醇发酵的影响。结果表明:添加微量的苄基紫精可以促进丁醇的产生,同时可强烈抑制丙酮的合成,丁醇体积分数由66.92%提高到82.35%。苄基紫精可促进菌株快速进入产溶剂期,发酵周期明显缩短,丁醇生产强度显著提高。7%玉米培养基中加入40 mg/L苄基紫精,丁醇产量最高达16.10 g/L,生产强度为0.37 g/(L.h),分别较对照提高10.96%和60.87%。在初始丁醇体积分数较低的条件下,苄基紫精对丁醇合成的促进作用更明显。

The effects of different electronic carriers on butanol fermentation by Clostridium acetobutylicum were investigated. Results showed that benzyl viologen on low concentrations could promote butanol production and reduce acetone production, and the butanol ratio increased from 66. 92% to 82.35%. Using benzyl viologen, C. acetobutylicum could enter solventogenesis rapidly and the fermentation period was shortened, thus butanol productivity was markedly increased. C. acetobutylicum produced 16. 10 g/L butanol with a productivity of 0. 37 g,/（ L· h） when grown in corn medium containing 40 mg/L benzyl viologen. Meanwhile, the butanol production and the productivity were 10. 96% and 60. 87%, higher than those of control. The promotion of the benzyl viologen on the butanol production was more active when the butanol ratio was lower.