老鹳草素对破骨细胞体外骨吸收功能的影响

Effects of Geraniin on Osteoclastic Bone-resorption Activity

目的观察老鹳草素(geraniin,Ge)对体外培养破骨细胞(OC)的形成及其骨吸收功能的影响.方法由1日龄SD大鼠四肢长骨分离OC,和象牙骨片共同培养,或直接接种于培养板中,分别采用抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)和甲苯胺蓝染色技术观察不同浓度的Ge对体外培养OC形成及OC性骨吸收功能的影响.结果 Ge呈浓度依赖性减少体外培养的TRAP染色阳性多核细胞即成熟破骨细胞(mature osteoclast,mOC)数目;与空白对照组比较,Ge各浓度组均使OC性骨吸收陷窝个数和面积减少、灰度变浅.结论 Ge减少体外培养OC的形成并抑制体外培养OC的骨吸收功能.

Objective To evaluate the effects of geraniin（Ge） on the generation of osteoclasts（OCs） and its bone-resorption activity in vitro.Methods OCs isolated mechanically from long bones of SD rats aged one-day were plated in 48-well plates directly or on ivory bone slices in 24-well plates for culture together with different concentrations of Ge.TRAP staining and toluidine blue staining were used to evaluate the generation of OC and bone resorption on ivory slices.Results Ge decreased the total numbers of multinucleated TRAP-positive osteoclasts in cultures.Ge decreased the number of resorption pits on slices,total areas of resorption pits on slices as well as the average luminance of pits.Conclusion Ge can inhibit the generation of OCs and the bone-resorption activity of osteoclasts in vitro.