摘要
目的观察细胞凋亡在SD大鼠Meckel's软骨上的变化,初步探讨细胞凋亡在Meckel’s软骨和下颌骨发育中的意义.方法用原位末端标记法(TUNEL)检测凋亡细胞在E13~19 dSD胎鼠的下颌骨发育过程中Meckel’s软骨中的变化.结果 E13~19 d在Meckel’s软骨嘴突软骨细胞有零星凋亡细胞出现,软骨膜上可观察到少量凋亡细胞.E17~19 d Meckel’s软骨前段骨化,近骨化处Meckel's软骨软骨细胞TUNEL染色"++".Meckel’s软骨前段远离骨化处和Meckel’s软骨中段Meckel’s软骨软骨细胞TUNEL染色"+".E17~19 d Meckel’s软骨前段和Meckel’s软骨中段Meckel’s软骨膜崩解,软骨膜细胞TUNEL染色"++",可见多个凋亡细胞聚集成团现象.骨化的Meckel’s软骨内也观察到凋亡细胞的出现,TUNEL染色"+".结论 Meckel’s软骨软骨膜细胞的消亡可能是以凋亡的形式消失,只有一部分Meckel's软骨软骨细胞通过凋亡的形式消失.
Objective To observe the changes of programmed cell death(apoptosis)in Meckel's cartilage during the development mandible of SD rats.Method The expression of apoptotic cells in Meckel's cartilage during the mandible development of E13-19 d SD fetal rat was detected by TUNEL.Results In 13 days to 19 days embryo,the sporadic apoptotic cells were detected in Meckel's mouth cartilage cells,and a small number of apoptotic cells were observed in Meckel's cartilage perichondrium.In the 17 days to 19 days embryo,the ossification of Meckel's front-end cartilage was found,and the TUNEL staining positive cells were near to the ossification site at the Meckel's cartilage chondrocytes.The weakly positive expressions of Meckel's cartilage chondrocytes for TUNEL staining were detected at the preceding away from the ossification and the middle of Meckel's cartilage.In 17 days to 19 days embryo,Meckel's cartilage perichondrium appeared disintegration at the front-end and the middle of Meckel's cartilage,and these cells were positive by TUNEL staining,some apoptotic cells gathered into a group.Apoptotic cells were also observed in the ossification of Meckel's cartilage,were weakly positive by TUNEL staining.Conclusions The demise of the Meckel's cartilage perichondrium cells is mainly in the form of apoptosis.Only part of Meckel's cartilage chondrocytes disappear through apoptosis.
出处
《昆明医科大学学报》
CAS
2012年第9期26-30,共5页
Journal of Kunming Medical University
基金
云南省教育厅科研基金资助项目(08C0108)
关键词
Meckel’s软骨
下颌骨
发育
凋亡
Meckel's cartilage
Mandible
Development
Programmed cell death(apoptosis)