P16^(INK4A)和NF-κB在不同亚型尖锐湿疣组织中的表达及意义

Detection and significance of P16^(INK4A) and NF-κB in different hypotype of condyloma acuminatum

目的:探讨感染不同亚型HPV的尖锐湿疣(CA)组织中P16INK4A和NF-κB的表达及意义。方法:用PCR技术对60例CA组织进行分型检测,再用免疫组化方法检测P16INK4A和NF-κB在HPV6/11阳性(低危型组)和HPV16/18阳性(高危型组)尖锐湿疣组织中的表达,同时用健康男性的包皮组织作对照。结果:在低危型组和高危型组尖锐湿疣组织中,P16INK4A的阳性率均为66.7%,对照组为0%。NF-κB在低危型组、高危型组和对照组中均有表达,阳性率为90%,三组的表达强度比较差异均有显著性意义(P<0.05);在尖锐湿疣组织中P16INK4A和NF-κB两者呈正相关。结论:P16INK4A和NF-κB在不同亚型尖锐湿疣组织中均存在着表达,且表达的水平不同。P16INK4A和NF-κB的表达增强可能参与尖锐湿疣组织中细胞的增生与调控。

Objectlve：To study the expression and significance of P16INK4A and NF-κB in different hypotype of condyloma acuminatum （ CA ）. Methods： PCR was used to detect different type HPV in 60 patients with CA, immunohistochemisty was used to tested the expressions of P16INK4A and NF-κB in 30 cases of high-risk type CA and 30 cases of low -risk type CA, and 25 cases of normal human foreskin tissues Results： The positive rate of P16INK4A was 66.7% in high-risk group and low -risk group CA, while 0% in the control. The positive rate of NF-KB was respectively 90% in three groups.The expression intensity of P16INK4A and NF-KB had statistical differences in three groups, and spearman related analysis between P16INK4A and NF-KB was correlation （P〈0.05） . Conclusion： The different over-expression P16INK4A and NF-KB in different hypotype of CA may be related to the degree of proliferation and regulation of CA.