蒙药吉如很-阿嘎如-8对盐酸肾上腺素致小鼠心律失常肝肾功能的影响

Influence of Mongolian Medicine Jiruhen-Agaru-8 on the Hepatic and Renal Functions of Mice with Arrhythmia Induced by Epinephrine Hydrochloride

目的:观察吉如很-阿嘎如-8对盐酸肾上腺素致小鼠心律失常模型小鼠肝肾功能的影响.方法:取小鼠60只,雄性.按体重随机分为空白对照组、模型组、大剂量组、中剂量组、小剂量组、阳性对照组等6组.每组10只.从实验的第一天开始,给药组大、中、小三个剂量组灌胃相应剂量吉如很-阿嘎如-8、0.5%CMC-Na药物溶液,模型组和空白对照组灌胃等容量的0.5%CMC-Na溶液,阳性对照组灌胃相应剂量的盐酸普罗帕酮片0.5%CMC-Na药物溶液,每天1次,共10天.末次给药后1h,尾静脉注射盐酸肾上腺素0.15ml/20g.观察每只小鼠心电图变化.观察10min.测完心电图后断头取血,3000r/min,离心15min,分离血清,待测相关指标;取心脏,制成10%的匀浆液,测定相关指标.结果:与空白组相比,各组血清和组织AST活性均降低,差异显著(分别为P<0.001,P<0.01,P<0.05.)模型组和大剂量组组织ALT活性明显降低,差异显著(分别为P<0.05,P<0.01.)与空白组相比,模型组组织URE活性明显升高,差异显著(P<0.001.)与模型组相比,吉如很阿嘎日-8大中小三个剂量组和阳性对照组组织URE活性明显降低,差异显著(分别为,P<0.001,P<0.01.)结论:盐酸肾上腺素对小鼠肾脏有一定损伤;吉如很阿嘎日-8有一定的保护肾脏功能.

Objective： To study the influence of Jiruhen-Agaru-8 on the hepatic and renal functions of mice with arrhythmia induced by epinephrine hydrochloride.Methods： 60 male mice were randomly divided into control,model,large-dose,medium-dose,small-dose and positive control groups（each group including 10 mice） according to their body weights.From the first day,mice in medication groups（consisting of large-dose,medium-dose and small-dose groups） were given Jiruhen-Agaru-8 in 0.5% CMC-Na solution with different doses via intragastric administration.Mice in model and control groups took the same volume of 0.5% CMC-Na solution as ones in medication groups.Mice in positive control group had the certain dose of propafenone hydrochloride.All mice took once a day and for total 10 days.Within 1h after the last administration epinephrine hydrochloride was injected via caudal vein with 0.15ml/20g.The electrocardiogram of each mouse was recoded within 10min.Then mice were decapitated to collect blood samples.Blood samples were centrifugated at 3000r/min and for 15min to obtain serum.Hearts of mice were prepared to get 10% tissue homogenate.Results： Compared with mice in control groups,mice’s AST activity in serum and tissue