基于药代动力学的蒙药地格达-4味汤配伍规律研究

Study on the Combination of Mongolian Medicine Digeda-4 Wei Tang Based on Pharmacokinetics

目的:通过蒙药"地格达—4味汤"中獐芽菜苦苷在大鼠体内的药代动力学参数的变化,验证地格达-4味汤组方的合理性和可行性.方法:将急性肝损伤模型大鼠分为蒙药地格达-4味汤组、君药+臣药组、君药+佐药组、君药+使药组等4组,分别灌胃相应药液,在不同时间点取血,分离血清,以HPLC法测定各血清样品中獐牙菜苦苷的含量,并计算药代动力学参数,比较方剂和拆方后三个药对的药代动力学参数,判定方剂组方的合理性.结果:含地格达-4味汤血清中獐牙菜苦苷含量在不同给药时间点均明显高于三个药对组含量.参数中地格达-4味汤Vd、T1/2、Cmax高于三个药对.结论:初步认为地格达-4味汤组方有一定的合理性和科学性.

Objective：To study the changes in pharmacokinetics of Swertiamarin in Mongolian Medicine Digeda-4 Wei Tang in rat model of acute liver injury and evaluate the reasonable and feasible formulation of Digeda-4 Wei Tang.Methods： Rats with acute liver injury were divided into Digeda-4 Wei Tang group,sovereign drug＋minister drug group,sovereign drug＋assistant drug group,sovereign drug＋envoy drug group,After intragastric administration to rat model,blood samples were collected at different time points and the serum was separated by Centrifuge.A high-performance liquid chromatographic method was developed to determine the content of Swertiamarin and calculate the parameter of pharmacokinetics.By comparing the pharmacokinetics of prescription and three couplet medicines via formula components analysis,rationality of prescription was evaluated.Results：The content of Swertiamarin in Digeda-4 Wei Tang was higher than that in other three groups at different time points.Vd,T1/2,Cmax in Digeda-4 Wei Tang is also higher than those in other three groups.Conclusion：Digeda-4 Wei Tang was initially considered as a reasonable and scientific prescription.