大鼠牙胚体内种植实验

Implantation of rat tooth germs in vivo.

目的:观察牙胚在肾被膜、大网膜和皮下的生长发育情况。方法:分离出生后(PN)7～8 d的SD大鼠下颌第一磨牙完整牙胚,分别种植到成年SD大鼠肾被膜、大网膜和皮下,4周后收获种植牙胚并进行组织学观察。结果:牙胚在肾被膜、大网膜和皮下均发育出牙根和牙周组织。结论:除了牙槽窝外,体内多个部位的微环境均可满足牙根和牙周组织生长发育的需要。

Objective：To observe the growth of tooth germs in renal capsule,omentum and subcutaneous tissue. Method： Mandibular first molar germs of postnatal 7 ~8 days SD rat were separated and implanted into renal capsule, omentum and subcutaneous tissue Of adult SD rat. After 4 weeks, the implanted germs were harvested and analysed histologically. Result： Development of tooth root and periodontium was observed in the implanted germs. Conclusion： Besides tooth socket, the microenvironment of other sites in vivo can support the growth of tooth root and periodontium.