不同花生品种响应干旱胁迫后叶片内ABA与AhNCED1的分布

Distribution of ABA and AhNCED1 in Peanut Leaves of Different Drought Resistant Cultivars Subjected to Drought Stress

以粤油7号和汕优523两个不同抗旱性品种为材料,研究响应干旱胁迫后叶片ABA(abscisic acid,脱落酸)和AhNCED1(Arachis hypogaea nine-cis-epoxycarotenoid dioxygenase)的分布以及含量变化。结果表明,两种花生品种响应干旱胁迫后叶片的维管组织中ABA分布增强且含量增加,AhNCED1蛋白分布也增强;且在水分胁迫初期粤油7号花生AhNCED1蛋白分布强于汕优523,其体内ABA分布水平也高于汕优523;经ABA生物合成抑制剂Naproxen处理后,两种花生叶片ABA分布减弱,但粤油7号叶片维管组织中ABA分布水平仍高于汕优523。结果表明维管组织是干旱胁迫下花生叶片中ABA和AhNCED1分布的主要区域,且粤油7号花生抗旱性强可能与其体内AhNCED1和ABA的分布量较高有关。

In this study, the changes of ABA distribution and ABA content in leaf of two species of the peanut （Yueyou 7 and Shanyou 523） were researched through the immunoenzyme localization and ELISA methods. The results showed that the ABA distribution and ABA content in peanut leaf were increased after drought stress. The synthesis of ABA and AhNCED1 in the leaves ofYueyou 7 appeared more quickly than that in the susceptible cultivar （Shanyou 523）. Furthermore, AhNCED1 transcript and protein in Yueyou 7 were induced more than that in Shanyou 523, coinciding with higher ABA accumulation. After treated with Naproxen, ABA distribution were lower than that treated with PEG. These results showed that the vascular cambium is the main region of leaf in peanut response to drought stress, and the higher drought-resistant of Yueyou 7 peanut may be relate to the more distribution of ABA and AhNCED1 in leaf.