摘要
[ Objective ] This study aimed to analyze the genetic diversity of turf bamboo species by using ISSR molecular marker technology. [ Method] Excellent turf bamboo species imported from France and domestic ornamental turf bamboo species were used as experimental materials for ISSR analysis, cluster analysis was conducted on 10 species of turf bamboo materials based on the obtained ISSR molectfiar ma^kers. [ Result] A total of 201 clear bands with good repeatability and high polymorphism were amplified with 21 ISSR primers, with a polymorphism rate of 93.1% ; similarity coefficients between different turf bamboo species ranged from 0.275 to 0.571, with an average similarity coefficient of 0. 357 ; according to the results of ISSR markers, 10 different ornamental turf bamboo species were di- vided into three categories by using UPGMA cluster analysis method. [Conclusion] Turf bamboo with different sources had relatively high genetic diversity, this study had provided theoretical and technical basis for the breeding, cultivation and vromotion of ornamental turf bamboo.
[ Objective ] This study aimed to analyze the genetic diversity of turf bamboo species by using ISSR molecular marker technology. [ Method] Excellent turf bamboo species imported from France and domestic ornamental turf bamboo species were used as experimental materials for ISSR analysis, cluster analysis was conducted on 10 species of turf bamboo materials based on the obtained ISSR molectfiar ma^kers. [ Result] A total of 201 clear bands with good repeatability and high polymorphism were amplified with 21 ISSR primers, with a polymorphism rate of 93.1% ; similarity coefficients between different turf bamboo species ranged from 0.275 to 0.571, with an average similarity coefficient of 0. 357 ; according to the results of ISSR markers, 10 different ornamental turf bamboo species were di- vided into three categories by using UPGMA cluster analysis method. [Conclusion] Turf bamboo with different sources had relatively high genetic diversity, this study had provided theoretical and technical basis for the breeding, cultivation and vromotion of ornamental turf bamboo.
基金
Supported by National Public-benefit Research Institute Fund(CAFINT2008C12)
