不同酶解法水解豆粕蛋白的比较研究

Comparative Study of Soybean Protein Hydrolysates Prepared in Different Ways

为研究不同的酶解方式对豆粕蛋白酶解过程及产物的影响,采用Alcalase蛋白酶、Protex.7L蛋白酶对豆粕蛋白进行单酶、双酶同步和双酶分步水解,以水解度、蛋白质利用率、多肽得率、寡肽得率为指标对酶解过程进行分析。结果表明采用先加Alcalase蛋白酶后加Protex.7L蛋白酶组合水解豆粕蛋白的效果最佳,最终水解液中水解度可达23.5%,蛋白质利用率、多肽得率、寡肽得率分别为68.7%、51.0%、21.4%。双酶分步水解的效果优于单酶水解和双酶同步水解;酶的加入顺序对豆粕的酶解过程有一定的影响。

Comparative Studies on the effect of hydrolysis processes and products ot soybean proLem by different enzymic hydrolysis. In the present study, Alcalase protease and Protex.7L protease were utilized to hydrolyze soybean protein in different ways, such as single-enzyme hydrolysis and one-step and stepwise double-enzyme hydrolysis. After degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield were measured to comparatively study soybean protein enzymolysis in different hydrolysis way. It was shown that the optimum hydrolysis way was sequential hydrolysis with Alcalase protease followed by Protex.7L protease. Under this way, the degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield reached 23.5%, 68.7%, 51.0%, 21.4%. The stepwise double-enzyme hydrolysis was superior to single-enzyme hydrolysis and one-step double-enzyme hydrolysis, and that there was obvious relation between hydrolysis process of soybean protein and the order of adding enzymes.