经颈内动脉移植骨髓间充质干细胞的安全性研究

Intraarterial injection of bone marrow mesenchymal stem cells： a safety study

目的 探讨骨髓间充质干细胞是否可以安全的通过颈内动脉移植。方法 密度梯度离心法常规体外培养骨髓间充质干细胞。应用脑血管造影技术，将导管超选择插入健康新西兰大白兔颈内动脉近端，通过导管经颈内动脉注入约1×10^6个间充质干细胞。术后24h行CT灌注成像检查及HE染色判断脑组织微循环的变化及有无微梗死灶发生。结果 成功经新西兰大白兔颈内动脉完成骨髓间充质干细胞移植术。术后24hCT灌注成像显示移植侧脑组织微循环灌注参数（脑血流量、脑血容量、平均通过时间、达峰值时间1变化与对照侧比较差异无统计学意义（P〉0．05），其中脑血流量较对照侧略有下降；HE染色显示脑组织无微梗死灶发生。结论 骨髓间充质干细胞可安全的经颈内动脉移植。

Objective To investigate whether mesenchymal stem cells derived from bone marrow （BMSCs） could be administered safely into the internal carotid artery. Methods Density gradient centrifugation was employed to extract the BMSCs. About 1×10^6 BMSCs were injected into the internal carotid artery of healthy New Zealand rabbits through cerebral angiography. After 24 h of injection, we checked the cerebral microcirculation with CT perfusion and possibly microinfarction with HE stainning. Results Successful administration of BMSCs into internal carotid artery of the rabbits was performed. No significant difference was found in perfusion parameters, including cerebral blood flow （CBF）, cerebral blood volume （CBV）, mean transit time （MTT）, and time to peak （TTP）, between transplanted hemisphere and contralateral hemisphere （P〉0.05）, despite a trend of slight decrease of CBF in the transplanted side. HE staining showed no microinfarctions in cortex-medulla junctional zone of the transplanted hemispheres. Conclusion BMSCs could be transplanted safely through internal carotid artery of the rabbits.