尿脱落细胞荧光原位杂交技术在尿路上皮癌诊断中的应用

Fluorescence in situ hybridization of urine exfoliative cells in diagnosis of urothelial carcinoma

目的:分析尿脱落细胞荧光原位杂交技术(FISH)在尿路上皮肿瘤诊断中的应用价值。方法:对205例临床疑诊尿路上皮肿瘤患者的尿脱落细胞进行FISH检测,并与组织病理学结果进行对照,总结FISH检测方法对尿路上皮癌诊断的敏感性与特异性。结果:153例尿路上皮癌、52例非尿路上皮癌患者在术前接受了尿脱落细胞FISH检测,术后均经病理检查确定诊断。FISH检测尿路上皮癌的敏感性为54.2%,特异性为94.2%。结论:尿脱落细胞FISH检测对于诊断尿路上皮癌具有高度的特异性。

Objective：To investigate the value of fluorescence in situ hybridization of urine exfoliative cells in the diagnosis of urothelial carcinoma. Method：The urine samples were collected from 205 patients with suspected urothelial neoplasms. The specificity and sensitivity of FISH were analyzed on the basis of pathological diagnosis. Result： One hundred and fifty-three patients with urothelial carcinoma and 52 cases of non-urothelial neoplasms received one FISH assay of their urine exfoliative cells before operation. The sensitivity of FISH examination of malignant epithelial tumor was 54.2%. The specificity of FISH on urothelial carcinoma was 94.2%. Conclusion：The FISH assay of the urine exfoliative cells shows high specificity for the detection of urothelial carcinoma.