血清SCCA水平及盆腔淋巴结HPV 16/18感染状况与宫颈鳞癌患者预后的关系

The relation of serum squamous cell carcinoma antigen level and pelvic lymph node HPV 16/18 infection with prognosis of cervical squamous cell carcinoma

目的探讨术前血清SCCA水平、盆腔淋巴结HPV 16/18感染状况与宫颈鳞癌患者预后的关系。方法ELISA法检测血清SCCA水平,实时荧光定量PCR法检测盆腔淋巴结HPV 16/18感染,分析二者与宫颈鳞癌患者3年生存率的关系。结果血清SCCA水平与盆腔淋巴结HPV 16/18感染相关(P<0.05)。35例宫颈鳞癌患者中位随访36个月(12～41月),死亡4例,3年总体生存率为85.0%。血清SCCA阴性者和阳性者的3年生存率分别为100%和69.2%;盆腔淋巴结HPV 16/18阴性者和阳性者的3年生存率分别为96.4%和57.1%;血清SCCA及盆腔淋巴结HPV 16/18阴性患者的3年生存率明显优于阳性者(P<0.05)。淋巴结转移、血清SCCA和淋巴结HPV16/18与宫颈鳞癌患者的预后相关。结论血清SCCA水平及盆腔淋巴结HPV 16/18感染状况与宫颈鳞癌患者预后相关,可作为宫颈鳞癌预后判断指标。

Objective To explore the relation of preoperative serum SCCA levels,pelvic lymph node HPV 16/18 infection in patients with cervical squamous cell carcinoma and their prognosis.Methods ELISA was performed to detect the SCCA level and real-time fluorescence quantitative PCR was utilized to analyze the positive rate of pelvic lymph node infection with HPV 16/18.The relation of SCCA level,HPV 16/18 positive rate and the three-year survival rate was investigated.Results The SCCA level was closely related with the infection of HPV 16/18 in pelvic lymph node（P0.05）.The mean follow-up time for 35 patients with cervical squamous cell carcinoma was 36 m（rang 12~41 months）.Four patients died and 3 year survival rate was 85.0%.The 3 year survival rates for patients with SCCA positive and negative were 100% and 69.2% respectively and that were 96.4% and 57.1% for patients with positive or negative pelvic lymph node infection with HPV 16/18.The 3-year survival rate for patients with negative SCCA and lymph node HPV 16/18 infection were obviously longer than that of positive ones（P0.05）.lymph node metastasis,SCCA level and pelvic lymph node HPV 16/18 infection status were associated with prognosis.Conclusions SCCA level and pelvic lymph node HPV 16/18 infection are associated with the prognosis of cervical squamous carcinoma patients and is a potential markers for cervical squamous carcinoma.