MMP-1、MMP-2在盆底器官脱垂疾病患者阴道组织中的表达及意义

Matrix metalloproteinases-1,-2 expression in vaginal tissues from women with pelvic organ prolapse

目的探讨基质金属蛋白酶与女性盆底器官脱垂性疾病的关系。方法选择20例盆底器官脱垂患者为研究组,20例非盆底器官功能障碍患者作为对照。RT-PCR法检测阴道前壁组织中MMP-1和MMP-2 mRNA的表达,明胶底物酶法测定阴道前壁组织中MMP-1、MMP-2的活性。结果脱垂患者阴道组织中MMP-1 mRNA的表达为0.68±0.06,显著高于对照组的0.41±0.03(P<0.01),pro-MMP-2在脱垂患者阴道壁组织酶活性为1.98±0.42,明显高于对照组的0.56±0.27(P<0.05)。结论 MMP-1使盆底组织胶原代谢分解增加,增加的MMP-1表达可能与盆底器官脱垂有关。

Objective To investigate the relation of Matrix metalloproteinase and female pelvic organ prolapse.Methods A total of 20 women under went pelvic reconstruction surgery for pelvic floor dysfunction or benign gynaecological disease were included in this study from January 2009 to May 2010.Vaginal tissue biopsies were obtained from patients with POP（n=20） and women without evidence of pelvic floor weakening（n=20）.Level of MMP-1,-2 and pro-MMP-2 in the vaginal tissue of women was measured.Results The expression of MMP-1 was significantly higher in vaginal tissue from women with POP（0.68±0.06） than that in control（0.41±0.03,P0.01）.In contrast,there was no difference in the expression of MMP-2 between women with and without POP.A more significantly increaseing in pro-MMP-2 expression was seen in vaginal tissue from women with POP（1.98±0.42） than that in control（0.56±0.27,P0.05）.Conclusions MMP-1 promotes the collagen degradation.Increased MMP-1 expression in the vaginal tissue is associated with urogenital prolapse.