β-catenin与小鼠毛囊衰老性变化相关

Role of β-catenin in mice hair follicle senescence

目的探讨β-catenin与毛囊衰老性变化的关系。方法取老龄与年轻小鼠处于毛囊周期各阶段的皮肤,RT-PCR和Western blot检测β-catenin在mRNA和蛋白水平的表达;IF检测比较β-catenin蛋白表达在毛囊中的组织学分布。结果老年鼠背皮的β-catenin表达相较处于同一毛囊周期阶段的年轻鼠皮肤在mRNA水平和蛋白质水平均有上调,出生后34个月的小鼠生长期皮肤中β-catenin蛋白的表达为0.9024±0.0167,显著高于出生后35 d的0.8012±0.0184。出生后34个月的小鼠静止期皮肤中β-catenin蛋白表达为0.6348±0.0241,显著高于出生后23 d的0.6348±0.0241(P<0.05)。IF检测显示β-catenin的表达集中在外根鞘和Bulge细胞,也可以观察到β-catenin的入核现象。结论β-catenin表达量上调,周期节律不明显,表达部位不同于年轻鼠可能是衰老相关性白发产生的原因之一。

Objective To investigate the relationship between β-catenin and mice hair follicle senescence.Methods The expression pattern of β-catenin in hair follicles of two age groups of C57 mice was eexamined by immunofluorescence,RT-PCR,and Western blot.Results Compaired to young mice,both β-catenin mRNA and protein expression levels were upregulated in old mice hair follicle at various stages of hair cycle.In the anagen skin of 34months old mice,β-catenin protein expressions were at the level of 0.9024±0.0167,significantly higher then the level in the anagen skin of 35 days old mice（0.8012±0.0184）.In the telogen skin of 34months old mice,β-catenin protein expressions were 0.6348±0.0241,significantly higher than the that in the telogen skin of 23 days old mice（0.6348±0.0241）（P0.05）.β-catenin protein distribution was localized at the up-portion of outer root-sheath and Bulge cells,while the nuclear expression was obviours.No apparente difference of the β-catenin expression pattern was observed in various stages of hair cycle in senile mice.Conclusion In old mice hair follicle,the changes of β-catenin expression pattern including the expression level,periodicity and localization may contribute to the senescent poliosis.