脐带血血浆对包皮成纤维细胞生长影响的实验研究

The effect of human umbilical cord plasma on the growth of human foreskin fibroblast

目的探讨人脐带血血浆(HUP)能否维持人包皮成纤维细胞(HFF)的生长。方法将脐血浆经盐析、透析等处理后,按10%体积成分培养HFF,并与同样浓度的胎牛血清(FBS)进行比较,观察细胞的贴壁速度、生长曲线、细胞周期及增殖能力。结果 HFF在含10%HUP的培养液中能长期生长,其生长生长形态、细胞增殖能力及特征都与在含同体积FBS的培养液中无显著差异。结论人HUP能维持HFF的长期生长。

Objective To investigate whether human umbilical cord plasma （HUP） can be used to culture human foreskin fibroblast （HFF） cell. Methods Surplus HUP were collected in this study. After being treated by salting out and diasysis, the HUP were used to culture HFF as 10% volume, and compared the effects with FBS. The features, growth curve, cell cycle and reproductive activity of HFF were observed. Results compared to fetal bovine serurn （FBS）, the morphous, reproductive activity and fibroblast characteristic of HFF cell cultured in HUP were not distinctively different. Conclusions HUP can be used to culture HFF.