大鼠缺血性急性肾损伤对肺脏超微结构的影响

Effects of ischemic acute kidney injury on ultrastructure of rat lung

目的观察大鼠缺血性急性肾损伤对肺脏细胞超微结构的影响。方法应用光学显微镜和透射电子显微镜技术以及立体计量学方法观察急性肾缺血60 min再灌注24 h后的肺脏。结果部分肺泡I型上皮细胞发生了肿胀性坏死,II型上皮细胞也出现了肿胀性坏死,细胞内大量空泡出现。嗜锇性板层小体消失,肺泡内皮细胞出现了凋亡的表现。肺内组织出现了中性粒细胞浸润。结论大鼠肾脏急性肾缺血损伤可引起肺细胞坏死与凋亡,其中坏死损伤为多见。此外,炎性细胞浸润也是不可忽略的症候。

[Abstract] Objective To observe the ultrastructural changes of damaged lung induced by ischemic acute kidney injury （IAKI） in rats. Methods Transmission Electron Microscopy and morphometric analysis were used in this study. Results Both type Ⅰ and Ⅱ alveolar cells undergoing necrosis could be seen in the lungs damaged by IAKI. Disappeared osmiophilic multilamellar bodies of damaged type Ⅱ cells could be found. In addition, endothelial cells undergoing apoptosis were also noted in the lungs damaged by IAKI. Neutrophilic leukocyte infiltration was evident in alveolar septum with IAKI. Conclusions Both necrosis and apoptosis are involved in the lungs damaged by IAKI, but necrosis is more evident than apoptosis. Leukocyte infiltration is also involved in the lungs of RIRI animals.