免疫性血小板减少症患者淋巴细胞亚群表达及其与血小板抗体、临床特征的相关性研究

STUDY ON THE EXPRESSION AND CORRELATION OF LYMPHOCYTE SUBSETS TO PLATELET ANTIBODY AND CLINICAL characteristic IN PATIENTS WITH IMMUNE THROMBOCYTOPENIA

目的研究免疫性血小板减少症(ITP)外周血淋巴细胞亚群表达及其相关性。方法用流式细胞术检测ITP及正常对照外周血淋巴细胞亚群,分析与血小板抗体、临床特征相关性。结果 ITP组CD19+显著增加,CD3+、CD3+CD4+、CD4+/CD8+、CD16+CD56+(NK细胞)显著降低(P<0.01);ITP组CD19+表达与其发病时间及就诊血小板数值呈正相关,NK细胞表达与其PAIgG水平呈负相关、与就诊血小板数值呈正相关(P<0.05)。结论除体液免疫参与ITP发病机制外,细胞免疫及NK细胞异常也与ITP发病有关。

Objective To investigate the expression and correlation of lymphocyte subsets to platelet antibody and clinical feature in patients with ITP. Methods The expression of lymphocyte subsets were measured by flow cytometry in 96 ITP patients and 45 normal subjects. Further, the correlation were ana- lyzed between the expression of lymphocyte subsets,platelet antibody and clinical feature. Results The ex- pression of CD19＋ was higher in ITP patients than in normal controls, the expression of CD3＋ , CD3＋ CD4＋ ,CD4＋/CD8＋ ,CD16＋ CD56＋ were lower in ITP patients than in normal controls （P〈0.01）. The expression of CD19 ＋ had positive correlation with the onset time and the number of platelet, the expression of CD16＋ CD56＋ had negative correlation with PAIgG and positive correlation with the number of platelet （P〈0.05）. Conclusion The disorder of the cellular immunity and NK cells may be involved in the patho- genesis of ITP except humoral immunity function.