利用杨树原生质体瞬时表达系统筛选杨生褐盘二孢菌效应因子蛋白

Identification of Marssonina brunnea f.sp.multigermtubi effector proteins using poplar protoplast transient expression system

效应因子蛋白在病原真菌侵染宿主植物过程中发挥着重要作用,效应因子的筛选和鉴定是目前研究的难点。笔者以杨生褐盘二孢菌全基因组测序结果为基础,根据真菌效应因子蛋白结构特征,预测出106个候选效应因子,并成功克隆其中25个。利用杨树原生质体瞬时表达系统对这25个候选效应因子蛋白进行细胞内功能筛选,鉴定出4个效应因子蛋白可以抑制杨树转录因子WRKY29启动子的活性,表明这4个效应因子蛋白在干扰植物免疫系统的信号传导途径中发挥重要作用。

Pathogenic fungi effector proteins play an important role in infecting their host plants.At present,identification and functional studies of the effector proteins are the emphasis and difficulty for disease resistance research in plants.Based on Marssonina brunnea f.sp.multigermtubi genome sequence and structural characteristics of fungal effector proteins,we predicted 106 candidate effectors,and 25 of them were successfully cloned.Then,we used poplar protoplast transient expression system to study the intracellular function of these 25 candidate effectors,and 4 of them were identified to inhibit the activity of poplar transcription factor WRKY29 promoter,which is involved in poplar immune system.The results indicated that these 4 effector proteins might play important roles in disturbing the signal transduction pathway of plant immune system.