期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

shRNA沉默RYBP基因表达对HL-60细胞增殖和凋亡的影响 被引量：1

原文传递
导出
摘要 Ring和YY1结合蛋白（RYBP）是生物进化过程中高度保守的转录抑制因子，目前认为是多梳（Polycombgroup，PcG）蛋白家族成员，与白血病、淋巴瘤、宫颈癌等肿瘤的发生、发展和预后密切相关。我们在前期实验中发现白血病患者骨髓单个核细胞（MNC）和白血病细胞系HL-60、SHI．1和K562细胞明显地高表达RYBP，且慢性髓性白血病（CML）患者治疗达到完全缓解时骨髓MNCRYBP表达量明显下降，复发时再升高。说明RYBP基因的表达异常与白血病的发生、预后等有关。我们试图通过慢病毒介导的RNA干扰技术沉默HL-60细胞RYBP表达，观察HL-60细胞增殖与凋亡，进一步探讨RYBP在白血病发生发展中的作用。
作者 王春丽 邓晖 张玉平 应逸 许艳丽 王顺清
机构地区 广州医学院附属广州市第一人民医院血液科
出处 《中华血液学杂志》 CAS CSCD 北大核心 2012年第10期874-876,共3页 Chinese Journal of Hematology
基金 国家自然科学基金（30871101） 广东省科技计划项目（20118031800289） 2009年度广州市留学人员专项资金作者单位：
关键词 HL-60细胞增殖 RNA沉默 基因表达 凋亡 骨髓单个核细胞 白血病患者 细胞系HL-60 慢性髓性白血病
分类号 R733.7 [医药卫生—肿瘤]
  • 相关文献

参考文献14

  • 1Sasaki D, Imaizumi Y, Hasegawa H, et al. Overexpression of Enhancer of zeste homolog 2 with trimethylation of lysine 27 on histone H3 in adult T-cell leukemia/lymphoma as a target for epigenetic therapy. Haematologica, 2011, 96:712-719.
  • 2Sanchez-Beato M, Sanchez E, Gonzalez-Carrer6 J,et al. Variability in the expression of polyeomb proteins in different normal and tumoral tissues. A pilot study using tissue microarrays. Mod Pathol, 2006,19:684-694.
  • 3Lando M, Holden M, Bergersen LC, et al. Gene dosage, expression, and ontology analysis identifies driver genes in the carcinogenesis and chemoradioresistance of cervical cancer. PLoS Genet, 2009, 5 : e1000719.
  • 4钟雯婷,王顺清,张玉平,许艳丽,应逸.RYBP在急性白血病中的表达[J].实用医学杂志,2011,27(18):3301-3303. 被引量：7
  • 5Arrigoni R, Alam SL, Wamstad JA,et al. The Polycomb-associated protein Rybp is a ubiquitin binding protein. FEBS Lett, 2006, 580:6233-6241.
  • 6Wang R, Taylor AB, Leal BZ, et al. Polycomb group targeting through different binding partners of RINGIB C-terminal domain. Structure 2010, 18:966-975.
  • 7Tavares L, Dimitrova E, Oxley D, et al. RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3. Cell, 2012, 148:664-678.
  • 8Chen D, Zhang J, Li M, et al. RYBP stabilizes p53 by modulating MDM2. EMBO Rep, 2009, 10: 166-172.
  • 9Taylor BS, Schuhz N, Hieronymus H, et al. Integrative genomic profiling of human prostate cancer. Cancer Cell, 2010, 18 : 11-22.
  • 10Danen-van Oorschot AA, Voskamp P, Seelen MC, et al. Human death effector domain-associated factor interacts with the viral apoptosis agonist Apoptin and exerts tumor-preferential cell killing. Cell Death Differ, 2004, 11:564-573.

二级参考文献12

  • 1Bejarano F, Gonzalez I, Vidal M, et al. The Drosophila RYBP gene functions as a Polycomb-dependent transcriptional repressor [J]. Mech,2005,122(6):1118-1129.
  • 2Schuettengruber B, Chourrout D, Vervoort M, et al. Genome regulation by polycomb and trithorax proteins [J]. Cell,2007, 128(4) :735-745.
  • 3Gonzalez I,Aparicio R, Busturia A. Functional characterization of the dRYBP gene in Drosophila [J]. Genetics,2008,179(7): 1373-1388.
  • 4Novak R L, Phillips A C. Adenoviral-mediated Rybp expression promotes tumor cell-specific apoptosis[J]. Cancer Gene Ther, 2008,15(4) :713-722.
  • 5Stanton S E, Blanck J K, Locker J, et al. Rybp interacts with Hippi and enhances Hippi-mediated apoptosis [J]. Apoptosis, 2007,12( 11 ) : 2197-2206.
  • 6Gonzalez I, Aparicio R, Busturia A. Functional characterization of the dRYBP gene in Drosophila [J]. Genetics,2008,179(3) : 1373-1388.
  • 7Pirity M K, Locker J, Schreiber-Agus N. Rybp/DEDAF is required for early postimplantation and for central nervous system development [J]. Mol Cell Biol, 2005, 25(16): 7193- 7202.
  • 8Sanchez-Beato M, Sanchez E, Gonzalez-Carrero J, et al. Variability in the expression of polycomb proteins in different normal and tumoral tissues. A pilot study using tissue microarrays [Jl. Mod Pathol, 2006, 19(5) : 684-694.
  • 9Sanchez-Beato M, Sanchez E, Garcia J F, et al. Abnormal PcG protein expression in Hodgkin's lymphoma. Relation with E2F6 and NFkappaB transcription factors [J]. J Pathol,2004, 204(5): 528-537.
  • 10Seitz V, Thomas P E, Zimmermann K, et al. Classical Hodgkin lymphoma shows epigenetic features of an abortive plasma cellular differentiation [J]. Haematologica,2011 Mar 10. [Epub ahead of print].

共引文献6

同被引文献14

  • 1Scott Gary K,Mattie Michael D,Berger Crystal E,Benz Stephen C,Benz Christopher C.Rapid alteration of microRNA levels by histone deacetylase inhibition. Cancer Research . 2006
  • 2Danen-van Oorschot A A A M,Voskamp P,Seelen M C M J,van Miltenburg M H A M,Bolk M W,Tait S W,Boesen-de Cock J G R,Rohn J L,Borst J,Noteborn M H M.Human death effector domain-associated factor interacts with the viral apoptosis agonist Apoptin and exerts tumor-preferential cell killing. Cell Death and Differentiation . 2004
  • 3Barry S. Taylor,Nikolaus Schultz,Haley Hieronymus,Anuradha Gopalan,Yonghong Xiao,Brett S. Carver,Vivek K. Arora,Poorvi Kaushik,Ethan Cerami,Boris Reva,Yevgeniy Antipin,Nicholas Mitsiades,Thomas Landers,Igor Dolgalev,John E. Major,Manda Wilson,Nicholas D. Socci,Alex E. Lash,Adriana Heguy,James A. Eastham,Howard I. Scher,Victor E. Reuter,Peter T. Scardino,Chris Sander,Charles L. Sawyers,William L. Gerald.??Integrative Genomic Profiling of Human Prostate Cancer(J)Cancer Cell . 2010 (1)
  • 4Toshinori Nakayama,Masakatsu Yamashita.??Critical role of the Polycomb and Trithorax complexes in the maintenance of CD4 T cell memory(J)Seminars in Immunology . 2009 (2)
  • 5Melinda K Pirity,Wei-Lin Wang,Louise V Wolf,Ernst R Tamm,Nicole Schreiber-Agus,Ales Cvekl.Rybp, a polycomb complex-associated protein, is required for mouse eye development. BMC Developmental Biology . 2007
  • 6Chen D,Zhang J,Li M,Rayburn E R,et al.RYBP stabilizes p53by modulating MDM2. EMBO Reports . 2009
  • 7Novak R L,Phillips A C.Adenoviral-mediated Rybp expression promotes tumor cell-specific apoptosis. Cancer Gene Therapy . 2008
  • 8Sasaki Daisuke,Imaizumi Yoshitaka,Hasegawa Hiroo,Osaka Akemi,Tsukasaki Kunihiro,Choi Young Lim,Mano Hiroyuki,Marquez Victor E,Hayashi Tomayoshi,Yanagihara Katsunori,Moriwaki Yuji,Miyazaki Yasushi,Kamihira Shimeru,Yamada Yasuaki.Overexpression of Enhancer of zeste homolog 2 with trimethylation of lysine 27 on histone H3 in adult T-cell leukemia/lymphoma as a target for epigenetic therapy. Haematologica . 2011
  • 9Zheng, Lixin,Schickling, Olaf,Peter, Marcus E.,Lenardo, Michael J.The Death Effector Domain-associated Factor Plays Distinct Regulatory Roles in the Nucleus and Cytoplasm. Journal of Biological Chemistry . 2001
  • 10Pirity Melinda K,Locker Joseph,Schreiber-Agus Nicole.Rybp/DEDAF is required for early postimplantation and for central nervous system development. Molecular and Cellular Probes . 2005

引证文献1

二级引证文献2

中华血液学杂志
2012年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部