Graves病患者外周血单个核细胞TIM-3、TIM-1及其相关基因mRNA的表达与临床意义

The increased express of TIM-l, TIM-3 and other association genes in peripheral blood mononuclear cells from patients with Graves disease

目的研究TIM-3、TIM-1、T—bet、GATA-3、IFN-γ、IL-4和Galectin-9基因在Graves病（GD）患者外周血的表达及其临床意义。方法通过实时定量RT-PCR分别检测70例Graves病患者及22例健康正常人外周血单个核细胞（PBMC）中TIM-3、TIM-1及其相关基因mRNA的表达情况，并分析TIM-3、TIM-1与其之间的关系。结果GD患者PBMC中TIM-3、TIM-1mRNA的表达异常增高，其突眼GD组TIM-3mRNA的表达要高于无突眼GD组，而TIM-1mRNA的表达却无明显差异。GD初诊患者，TIM-3mRNA的表达要显著高于复发组，但TIM-1mRNA的表达却恰恰相反。GD缓解患者，TIM-3mRNA的表达与健康对照组差异无统计意义，TIM-1mRNA的表达虽显著下降，但仍高于正常对照组。结论TIM-3、TIM-1可能参与了GD的发生、发展和晚期转归，TIM-3或（和）TIM-1有可能为治疗GD提供-个新的靶点。

Objective To detect the levels of the mRNA expression of TIM-3, TIM-1, T-bet, GATA-3, IFN-γ, IL-4 and Galectin-9 in the peripheral blood monocytes （PBMCs） of the patients with Graves disease（ GD）, and to explore their potential role in the pathogenesis of GD. Methods We used flu- orescence quantitative real-time reverse transcription-polymerase chain reaction to measure the mRNA ex- pression of TIM-3, TIM-1 and other associated genes in PBMCs of 70 patients with GD and 22 healthy con- trols. In addition, we analyzed the relationship of TIM-3, TIM-1 and other associated genes. Results The expression of TIM-3 and TIM-1 mRNA in the PBMCs from GD patients were abnormally higher, the GD pa- tients with Graves＇ ophthalmopathy group had significantly higher level of TIM-3 mRNA expression than that of GD patients without Graves＇ ophthalmopathy group, but no statistically significant difference was found in the expression of TIM-1 mRNA. Untreated GD patients had significantly higher level of TIM-3 mRNA ex- pression than that of GD patients in recurrence group, however the expression of TIM-1 mRAN was opposite. But no statistically significant difference was found in TIM-3 mRNA expression of recovery GD patient and healthy control group. Though the expression of TIM-1 mRNA was significantly decreased, it was still higher than that of the normal control group. Conclusion TIM-3 and TIM-1 may participate in the occurrence, de- velopment and turnover of GD. TIM-3 or TIM-1 may prove to be an important target for developing new drugs and treatments to GD.