摘要
目的 研究南京地区5岁及以下腹泻患儿人类杯状病毒感染的分子流行病学特点。方法2009年7月至2010年6月在南京儿童医院共采集1-59个月的腹泻患儿粪便标本300份，采用多重RT-PCR方法检测人类杯状病毒（诺如病毒、札如病毒）。结果300份标本中71份检出人杯状病毒，检出率23．67％。其中，诺如病毒67份，其中58份为GⅡ／4基因型（2006b亚型），8份为GⅡ／3基因型，1份为GⅡ／12基因型；扎如病毒4份，其中2份为GⅠ／1基因型，GⅠ／2和GⅡ／1基因型各1份。结论人类杯状病毒是南京地区腹泻婴幼儿中的主要病原体之一，主要的流行优势株是诺如病毒GⅡ／4基因型（2006b亚型）
Objective To study molecular epidemiological characteristics of human caliciviruses among infants and young children aged 0-5 years old with acute diarrhea in Nanjing. Methods Stool specimens were collected from 300 infants and young children less than 5 years old admitted with acute gastroenteritis to Nanjing Children＇s Hospital from July 2009 to June 2010. Human caliciviruses （including noroviruses and sapoviruses） were detected by RT-multiplex PCR. Results Human caliciviruses were identified in 71 cases from the 300 specimens and the detection rate was 23.67%. Out of these 71 positive samples, 67 cases were Norovirus including 58 of GⅡ/4 （2006b strain）, 8 of GⅡ/3 and 1 of GⅡ/12; 4 cases were Sapovirus including 2 of GⅠ/1, I of GI/2 and 1 of GⅡ/1, respectively. Conclusions Human calicivirus is one of the main pathogens of diarrhea in infants and young children in Nanjing. Norovirus GⅡ/4 genotype （2006b strain） was the most predominant strain.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2012年第10期924-927,共4页
Journal of Clinical Pediatrics
基金
南京市医学科技发展基金(No.ZKX09008)
江苏省卫生厅医学科技发展基金(No.H200949)
