水苏糖对新生大鼠NEC发病影响及其机制的研究

The study of effect of stachyose on pathogenesis of NEC and the mechanism in neonatal rat

目的建立新生SD大鼠坏死性小肠结肠炎(NEC)模型,探索水苏糖防治NEC的作用及其机制。方法 SD新生大鼠随机分为6组,每组6只,分别为正常对照组,NEC对照组,水苏糖高、中、低剂量治疗组,以及益生菌(主要为双歧杆菌)治疗组。除正常对照组新生大鼠继续与母鼠同笼饲养外,其余组新生大鼠出生48h后与母鼠分开,置入保育箱中采用鼠乳代用品人工喂养,并定期给予缺氧冷刺激,建立新生大鼠NEC模型。同时,NEC对照组大鼠灌服蒸馏水,水苏糖高、中、低剂量治疗组分别灌服不同剂量的水苏糖,益生菌治疗组灌服益生菌。于实验第4天处死大鼠并采血留取小肠标本,检测小肠组织内毒素(LPS)、血浆血小板活化因子(PAF)及肿瘤坏死因子-α(TNF-α)。结果与正常对照组大鼠比较,NEC对照组、水苏糖高、中、低剂量治疗组、益生菌治疗组的LPS、PAF、TNF-α水平均升高,差异有统计学意义(P均<0.01);与NEC对照组大鼠相比,中、高水苏糖组及益生菌组的LPS、PAF、TNF-α水平均降低,差异有统计学意义(P均<0.01),低水苏糖组与NEC对照组间的差异无统计学意义(P均>0.01)。结论 LPS、PAF、TNF-α水平与NEC的发病相关;水苏糖对NEC的治疗有益。

Objective To establish the necrotizing enterocolitis （NEC） model in the neonatal SD rats and to explore the effect of stachyose on NEC prevention and the mechanism. Methods The neonatal SD rats were randomly divided into 6 groups of 6 rats each, normal group, NEC control group, three stachyose treatment groups with high, medium or low dose stachyose, respectively, probiotics （consisting mainly of Bifidobacteria） treatment group. Except that the rats in normal group were still fed from their mothers, the rats in other groups were separated from their mothers at 48 h after birth, then put into the incubators with artificial feeding using the rat milk substitutes, and regularly stimulated with hypoxia and cold in order to establish neonatal rat NEC model. At the same time, rats in NEC control group were fed with distilled water, rats in three stachyose treatment groups were given high, medium or low doses of stachyose and rats in probiotics treatment group were fed with probiotics. On the 4th day of the experiment when all the rats were sacrificed, samples of blood and small intestine were taken. Endotoxin （LPS） in small intestinal tissue, plasma platelet activation factor （PAF） and tumor necrosis factor alpha （TNF alpha） were detected. Results Compared with normal group, the LPS, PAF and TNF alpha levels in NEC control group, three stachyose treatment groups and the probiotics group were higher, and the differences were statistically significant （all P〈0.01）. Compared with NEC control group, the LPS, PAF and TNF alpha levels in treatment groups with high or medium dose stachyose and probiotics were lower, and the differences were statistically significant （all P〈0.01）. There were no significant differences of the LPS, PAF and TNF alpha levels between low dose stachyose treatment group and NEC control group. Conclusions The LPS, PAF and TNF alpha levels are relatedto the pathogenesis of NEC, and the stachyose is beneficial in the treatment of NEC.