不同心理弹性水平个体在特里尔社会应激时主观紧张度、唾液α-淀粉酶和糖皮质激素浓度的变化

Individual subjective tension and salivary levels of α-amylase and glucocorticoid in college students with different levels of resilience in Trier social stress test

目的研究不同心理弹性水平个体在特里尔社会应激(TSST)时主观紧张度、唾液α-淀粉酶和糖皮质激素浓度的变化情况。方法采用心理弹性量表(CD-RISC)在某军医大学对160例大学生进行筛查,根据测验结果选取高心理弹性组(n=11)和低心理弹性组(n=11)共22例入组TSST试验,全部为男性,年龄为(20.16±0.77)岁,2组被试在文化、年龄和教育水平上无统计学差异(P>0.05)。按照TSST标准化试验程序进行,被试在试验前后的第1、10、25分钟时完成4次唾液样本和主观紧张度的采集。结果①与基线值相比较,高、低心理弹性组的主观紧张度在TSST试验后1 min时达到最高峰后逐渐降低,于25 min时降至最低;高、低心理弹性组[分别为(2.73±0.79)、(3.64±0.51)]在TSST试验后1 min时差异显著(P<0.01);②与基线值相比较,高心理弹性组唾液中sAA浓度在TSST试验后逐渐增高,25 min时显著增高[(436.24±79.97)μmol/L,P<0.01];低心理弹性组在TSST试验后1 min时浓度降低,之后逐渐增高,25 min时显著增高[(478.29±108.72)μmol/L,P<0.05];③与基线值相比较,高心理弹性组唾液中GC浓度在TSST试验后逐渐增高,25 min时显著增高[(1 003.03±259.26)μg/L,P<0.01];低心理弹性组在TSST试验后1 min时浓度降低,之后逐渐增高,但差异无统计学意义(P>0.05)。结论 TSST试验后,高心理弹性个体在急性应激时有更快的积极响应,应激后心境和激素水平恢复情况好于低心理弹性个体。

Objective To investigate the subjective tension,salivary levels of α-amylase（sAA） and glucocorticoid（GC） in the individuals with different levels of resilience in Trier social stress test（TSST）.Methods Connor-Davidson resilience scale（CD-RISC） was used in 160 students from a military medical university.According to the test results,22 individuals were selected,and 11 of them were assigned as high resilience group（n = 11） and the other 11 was low resilience group（n = 11）.All these 22 individuals were male,with an average age of 20.16 ± 0.77.The 2 groups had no significant difference in culture background,age and educational level（P〉0.05）.The test follows the TSST standard procedures for the 2 groups.Their saliva samples were collected before and in 1,10 and 25 min after the test.Their subjective tension was evaluated by themselves at the same time points.Results Compared with the baseline value,the subjective tension in both high and low resilience groups reached its peak in 1 min after TSST and then declined to the lowest in 25 min.The tension in 1 min after TSST had significant difference in the high and low resilience groups（2.73 ±0.79 vs 3.64 ±0.51,P〈0.01）.Compared with the baseline values,saliva levels of sAA and GC in the high resilience group was increased gradually after TSST experiment,and was significantly higher in 25 min（436.24 ±79.97 μmol/L and 1 003.03 ±259.26 μg/L,P〈0.01）.While the levels were decreased in 1 min after TSST in the low resilience group,and then the level of sAA was elevated gradually and reached the highest in 25 min（478.29 ± 108.72 μmol/L,P〈0.05）,but no significant difference was found in that of GC though it was also risen gradually（P〈0.05）.Conclusion After TSST experiment,in acute stress,high resilience individuals response faster,with better recovery in their mood and hormone levels after stress when compared with low resilience people.