辛伐他汀抑制大鼠炎症性肠病结肠纤维化结缔组织生长因子的表达

Inhibitory effect of simvastatin on CTGF expression in colonic fibrosis in rats with inflammatory bowel disease

目的探讨大鼠炎症性肠病(IBD)结肠纤维化结缔组织生长因子(CTGF)的表达及辛伐他汀对其影响。方法 32只SD大鼠均分为对照组、IBD模型对照组、辛伐他汀低剂量(5mg/kg)和高剂量(20mg/kg)干预组。21d后观察结肠病理组织学变化;RT-PCR和Western blot检测结肠Ⅰ型胶原、CTGF mRNA和蛋白表达。结果与对照组相比,模型组结肠黏膜及黏膜下层见大量炎症细胞浸润,纤维组织增生,Ⅰ型胶原和CTGF表达增多(P<0.01)。辛伐他汀干预后,病理组织学较模型组改善,Ⅰ型胶原和CTGF表达明显下降(P<0.05)。结论 CTGF参与结肠炎大鼠肠纤维化形成,辛伐他汀可能通过抑制CTGF的表达减轻IBD肠纤维化进展。

Objective To investigate the expression of connective tissue growth factor（CTGF） in colonic fibrosis in rats with inflammatory bowel disease（IBD） and intervention effect of simvastatin on it. Methods Thirty-two SD rats were equally divided into four groups of A（blank control）, B （model control）, C（treated with simvastatin 5 mg/kg daily for 21 days） and D（treated with simvastatin 20 mg/kg daily for 21 days）. Colonic histological changes were observed 21 days later and the mRNA and protein expressions of collagen types I and CTGF in the colon were detected with RT-PCR and Western blotting. Results Compared with group A, the colonic mucosa and submucosa showed inflammatory infiltration of the ceils, fibroplasia, and increased expression of collagen types I and CTGF in colonic tissue in group B（P〈0. 01） ,which were all improved in groups of C and D after simvastatin intervention（P〈0. 05）. Conclusion CTGF may play important roles in the development of intestinal fibrosis in colitis. Simvastatin can attenuate the progression of intestinal fibrosis of IBD by inhibiting the expression of CTGF.