人卵巢组织中HBsAg、HBV DNA的表达与HBV垂直传递的相关性

Relationship between HBsAg,HBV DNA expression in human ovary tissues and vertical transmission of hepatitis B virus

目的研究人卵巢组织中乙型肝炎病毒表面抗原(HBsAg)和HBV DNA的表达与垂直传递的相关性。方法对33例血清HBsAg和HBeAg均阳性的孕妇,采用免疫组化SP法检测卵巢组织中HBsAg,荧光定量PCR法检测卵巢组织中HBV DNA的表达。分析HBsAg和HBV DNA的表达与垂直传递的相关性。结果 33例孕妇中,7例卵巢组织HBsAg阳性,婴儿宫内感染率42.85%(3/7);其中的2例在卵泡中呈阳性表达,卵泡阳性的宫内感染率100%(2/2)。卵巢组织HBV DNA阳性12例(HBV DNA定量>1.0×106copies/ml),婴儿宫内感染率33.33%(4/12)。宫内感染婴儿较非宫内感染婴儿母亲卵巢组织中HBV DNA的表达水平和阳性率均明显升高,但差异无统计学意义。结论 HBV可感染人卵巢组织,且有可能通过卵细胞垂直传递至子代。

Objective To investigate the relationship between HBsAg and HBV DNA expression in human ovary and vertical transmission of hepatitis B virus （HBV）. Methods HBsAg and HBV DNA in the ovarian tissues were detected by immunohistochemisty and fluorescent quantitative PCR in 33 pregnant women who were positive for HBsAg and HBeAg. The relationship between HBsAg and HBV DNA expression in the ovary and vertical transmission of HBV was analyzed. Results Of 33 cases, 7 ovarian samples were positive in HBsAg, of whom 3 （42. 85%） infants had intrauterine HBV infection. In 7 HBsAg positive wemen, 2 cases were positive in ovarian folliclestroma and had 100% intrauterine HBV infection. Of 33 cases, 12 ovarian samples with the level of HBV DNA〉 1.0 X 106 copies/ml were positive in HBV DNA, of whom 4 （33.33%） infants had intrauterine HBV infection. The positive rate and expression level of HBV DNA in maternal ovary were higher in the infants having intrauterine HBV infection than those without（P〉0. 05）. Conclusion HBV can infect the human ovary and may transmit to fetus via the ovum.