中华蜜蜂嗅叶胚后发育过程中的神经胶质

Glial patterning during the postembryonic development of the olfactory lobe of the honeybee(Apis cerana cerana)

本研究通过形态解剖、免疫组织化学等技术,对中华蜜蜂Apis cerana cerana Fabricius工蜂嗅叶胚后发育中神经胶质的模式进行了比较研究。结果表明:神经胶质在蜜蜂嗅叶的发育过程中起着重要的作用,它们在嗅叶发育早期就划定了神经纤维网的边界;在嗅觉神经轴突进入嗅叶之前,对神经轴突进行"分选",并引导它们进入嗅叶特定的区域,形成神经纤维球;它们不仅规定了神经纤维网的边界和区域,还为神经纤维网提供内部的分隔。中华蜜蜂神经胶质增殖的高峰期集中在幼虫发育末期和预蛹期。

Glial cells have several functions during the development of the nervous system. The characteristics of glial patterns during the postembryonic development of the olfactory lobes of Apis cerana cerana Fabriclus were investigated using comparative anatomy and BrdU immunohistochemistry. Th＇e results show that a continuous layer of glial cell bodies defined the boundaries of all growing neuropiles in early larvae. Just before the olfactory receptor neuron axons entered the antennal lobe, they encountered the so - called sorting - zone glia. Here, axonal contact lead to a pause in the growth cone＇ s advance and a subsequent change in growth cone morphology. Finally this enabled the sorting of olfactory receptor neurons axons into discrete fascicles, which terminated in specific glomeruli. Proliferation of glial cells peaked from the end of the final larval instar stage to the prepupal stage.