中华通草蛉越冬成虫肠道可培养好氧细菌多样性分析

Diversity of intestinal aerobic bacteria in overwintering adults of Chrysoperla sinica

本研究以昆虫微生态学原理为指导探究中华通草蛉Chrysoperla sinica(Tjeder)的肠道菌群构成,为中华通草蛉的营养生理研究及人工繁殖利用提供理论依据和技术基础。采用无菌解剖及纯培养分离检测技术,从雌、雄成虫肠道环境中分离、纯化、培养,获得10个细菌菌株,对菌体形态、染色反应、培养形状、生理生化反应进行系统研究。结果表明,上述10个菌株分别属于摩根氏菌属(Morganella)、短小芽孢杆菌(Bacillus pumilus)、地杆菌属(Terrabacter)、爱德华氏菌属(Edwardsiella)、色杆菌属(Chromobacterium)、热黄杆菌(Flavobacterium thoupophilum)、放线杆菌属(Actinobacillus)、棍状杆菌属(Clavibacter)、邻单胞菌属(Plesiomonas)、预研菌属(Yokenella);越冬期雌、雄成虫肠道细菌的种类和数量存在较大差异,可能与雌、雄成虫不同的营养和生理需求有关,两者关系还需进一步研究。

An important natural enemy of many pests, Chrysoperla sinica （Tjeder） has a wide distribution and strong predatory capacity. The primary objective of this study was to explore the microbial ecology of the intestinal bacteria of C. sinica and provide theoretical and technological references for research on its nutritional physiology and husbandry. Ten bacterial strains were obtained from different genders of C. sinica by means of pure cultivation. These were identified on the basis of morphology, staining, cultivation characteristics, physiological and biochemical reactions as, Morganella, Bacillus pumilus, Terrabacter , Edwardsiella, Chromobacterium, Flavobacterium thoupophilum, ActinobaciUus, Clavibacter , Plesiomonas and Yokenella. Both the type and quantity of intestinal bacteria differed between adult C. sinica. This may be related to differences in the nutrition and physiology of different genders but further research is required to confirm this.