TRAIL联合阿霉素和IFN-γ对人骨肉瘤细胞TRAIL相关受体的影响

The Expression of TRAIL Receptors in MG-63 Cells after Different Combinations of TRAIL,Adriamycin and IFN-γ Treatment

目的:探讨TRAIL联合阿霉素和IFN-γ对人骨肉瘤细胞凋亡的作用及可能机制。方法:采用MTT法检测不同浓度及不同时间下TRAIL的抗癌活性。用流式细胞仪检测TRAIL、阿霉素和IFN-γ单独及联合应用后人骨肉瘤MG-63的凋亡率。RT-PCR法检测DR5及Yin Yang1(YY1)mRNA在加药前后的表达。结果:TRAIL、阿霉素、IFN-γ三者联合作用于MG-63细胞后,能显著增强对MG-63的杀伤、抑制增殖及诱导凋亡作用,明显高于单独应用TRAIL组或阿霉素组(P<0.05)。结论:TRAIL、阿霉素、IFN-γ三者联用能显著提高骨肉瘤细胞MG-63生长抑制和诱导凋亡作用,其机制可能与DR5mRNA表达上调及Yin Yang1(YY1)mRNA表达被抑制有关。

Objective： To investigate the effect and potential mechanism of combined TRAIL,adriamycin（ADM） and IFN-γ on apoptosis of human osteosarcoma cells in vitro.Methods：Osteosarcoma cells were cultured with TRAIL at different concentrations and different time periods.Methyl thiazolyl tetrazolium（MTT） assay was used to measure the anti-cancer activity of TRAIL.Flow cytometry was used to detect the apoptosis induced by TRAIL,ADM alone and TRAIL,ADM and IFN-γ combined.Expression of DR5 and Yin Yang1（YY1） mRNA was examined by RT-PCR.Results：The combination of the three treatments induced significantly higher apoptosis and cytotoxicity than exposure to TRAIL,ADM or IFN-γ alone,but the combination of two of the three agents did not show synergistic effect.Conclusion：There might be a synergistical interaction between paclitaxel in terms of cytotoxicity and its mechanisms might be involved in the up-regulation of DR5 gene and the down-regulation of YY1.