喉癌组织中核表皮生长因子受体与Aurora-A激酶的表达及其临床意义

Expression and clinicopathological significance of nuclear epidermal growth factor receptor,Aurora-A kinase in laryngeal squamous cell carcinoma

目的分析喉癌组织中核表皮生长因子受体(nuclear EGFR)、Aurora-A激酶的表达及其在评价喉癌生物学行为和预后中的价值。方法回顾性分析50例喉癌患者临床病理资料及随访结果,免疫组化法检测肿瘤组织中核表皮生长因子受体(EGFR)、Aurora-A激酶的表达。结果 50例喉癌患者的组织中,核EGFR、Aurora-A激酶阳性表达率分别为50%(25/50)、58%(29/50),均高于癌旁组织(P<0.05);核EGFR的表达与喉癌的原发灶分期、区域淋巴结转移、复发有关(P均<0.05);Aurora-A的表达与原发灶分期、区域淋巴结转移有关(P值均<0.05);核EGFR与Aurora-A表达的相关分析差异有统计学意义(r=0.446,P<0.05);Kaplan-Meier生存分析显示患者3年和5年生存率分别为84.0%和67.8%,Log-rank检验显示原发灶分期、区域淋巴结转移、核EGFR、Aurora-A的表达和患者预后有关(P<0.05)。Cox比例分析模型分析显示原发灶分期、区域淋巴结转移、核EGFR表达为影响喉癌患者预后的独立危险因素(P<0.05)。结论在喉癌发生和发展过程中存在核EGFR、Aurora-A激酶的异常表达,并与肿瘤的生物学行为和预后有关。

Objective To investigate expressions of nuclear EGFR and Aurora-A kinase in laryngeal squamous cell carcinoma, and to explore their associations with the clinicopathological feathers. Methods Expressions of nuclear EGFR and Aurora-A kinase were analyzed in 50 laryngeal squamous cell carcinoma samples and 10 normal tissues ad- jacent to the carcinomas by immunohistochemistry. Results Nuclear EGFR and Aurora-A kinase were up-regulated in most laryngeal squamous cell carcinoma samples but not in normal tissues. The difference of weighted scores be- tween these two groups was significant（ P 〈 0.05 ）. Although there were no correlations between expression of nucle- ar EGFR and some tumor parameters, such as age, gender, tumor site, expression of nuclear EGFR was significantly associated with tumor T staging（ P 〈 0.05 ） , lymph node metastasis（ P 〈 0.05 ） and relapse（ P 〈 0.05 ）. There were no correlations between expression of Aurora-A kinase and some tumor parameters, such as age, gender, tumor site, relapse （ P 〉 0.05 ）, while expressions of Aurora-A kinase was significantly associated with T staging （ P 〈 0.05 ） , lymph node metastasis （ P 〈 0.05 ）. In laryngeal squamous cell carcinoma tissues, there were positive correlation be- tween expressions of nuclear EGFR and Aurora-A kinase （ P 〈 0.05 ）. Kaplan-Meier survival analysis showed the 3, 5-year survival rates of high nuclear EGFR and Aurora-A kinase were significantly lower than in the contrary groups （ 84.0 %, P = 0.020 ; 67.8 %, P = 0.006, respectively）. Log-rank test demonstrated that patients with high tumor T staging, lymph node metastasis, and expressions of nuclear EGFR and Aurora-A kinase had a poor outcome （ P 〈 0.05 ）. While Cox multiple factors analysis showed that tumor T staging, lymph node metastasis and nuclear EGFR were independent prognostic factors of laryngeal squamous cell cancer（P = 0.045, 0. 018, 0.088, respectively）. Conclusion Nuclear EGFR and Aurora-A kinase are associated with biological behaviors and prognosis of laryngeal squmous cell cancer.