DNA倍体和Ki67对喉鳞状细胞癌预后的预测价值

Prognostic value of DNA ploidy and Ki67 in squamous cell carcinoma of larynx

目的探讨DNA倍体及细胞增殖抗原标记物Ki67对喉鳞状细胞癌预后预测的价值。方法采用Feul-gen染色和免疫组化方法,对298例喉鳞状细胞癌石蜡标本DNA倍体情况及Ki67表达进行分析。结果喉鳞状细胞癌组织中存在着DNA非整倍体肿瘤和DNA二倍体肿瘤;DNA非整倍体肿瘤中Ki67表达高于DNA二倍体肿瘤中的表达,结果差异有统计学意义(P<0.05);进一步经COX回归多因素分析发现喉鳞状细胞癌中DNA倍体的不同和Ki67表达的高低是影响肿瘤死亡的独立因素(P=0.003,P=0.007)。结论在喉鳞状细胞癌中,DNA倍体和Ki67表达水平与颈部淋巴结转移、肿瘤复发及患者生存率相关,是判断喉鳞状细胞癌患者预后估计的提示性依据。

Objective To investigate the prognostic value of DNA ploidy and Ki67 in squamous cell carcinoma of larynx. Methods The DNA ploidy samples of 298 squamous cell carcinoma of larynx were analyzed by Feulgen stained imprints and the expression of Ki67 was analyzed in those samples with immunohistochemistry. Results There were two groups, aneuploidy tumors and diploidy tumors, identified in squamous cell carcinomas of larynx. The expression of Ki67 in aneuploidy tumors were higher than those in diploidy tumors （ P 〈 0.05）. By Cox regression of multivariate analysis, DNA ploidy （P = 0.003 ） and Ki67 （P = 0.007 ） were shown to be independent prognostic fac- tors for survival of squamous cell carcinoma of larynx. Conclusions DNA ploidy and Ki67 are related to the displace- ment, recidivation and existence of squamous cell carcinoma of larynx and they might serve as prognostic factors.