主动免疫治疗对反复胚胎植入失败患者CD4^+CD25^+调节性T细胞表达的影响

Effect of active immunotherapy on CD4^+ CD25^+ T regulatory cells in recurrent implantation failure

目的探讨淋巴细胞主动免疫治疗对反复胚胎植入失败(RIF)患者外周血CD4^+CD25^+调节性T淋巴细胞(CD4^+CD25^+Treg)表达的影响。方法选择在郑州大学第三附属医院生殖中心就诊的30例RIF患者(RIF组),采用荧光标记流式细胞分析技术,检测其淋巴主动免疫治疗前、后2周的外周血CD4^+CD25^+ Treg表达,并选择同期正常未妊娠妇女20例作对照(对照组)。结果 (1)RIF组治疗前CD4^+CD25^+ Treg占CD4^+T细胞的比例显著低于对照组,差异有统计学意义(P<0.05);(2)RIF组治疗后CD4^+CD25^+ Treg的表达率明显高于治疗前,差异有统计学意义(P<0.05)。结论 RIF的发生可能与CD4^+CD25^+ Treg的表达下降有关;淋巴细胞主动免疫治疗可上调CD4^+CD25^+ Treg的表达,调控母胎免疫耐受,有利于胚胎植入成功。

Objective： To investigate the effect of active immunotherapy on CD4＋ CD25＋T regulatory cells （CD4＋ CD25＋ Treg） in women with recurrent implantation failure （RIF）. Methods： Thirty RIF patients from the Third Affiliated Hospital of Zhengzhou University were enrolled in our study. The percentage of CD4＋ CD25＋ Treg in peripheral blood was measured by doublelabel flow cytometric analysis before and after active immunotherapy. Twenty normal non-pregnant women were selected as control. Results： （1） The expression of CD4＋ CD25＋ Treg was significantly lower in RIF women than that in control; （2） The expression of CD4＋ CD25＋ Treg was increased significantly in RIF women after active immunotherapy. Conclusions： The results suggested that down-regulation of CD4＋ CD25＋ Treg might be associated with RIF, and active immunotherapy could enhance the percentage of CD4＋ CD25＋ Treg in periphera blood, which could maintain maternal-fetal tolerance for embryo implantation.