摘要
以苏云金芽孢杆菌库斯塔克亚种(Bacillus thuringiensis subsp.kurstaki)8010为研究材料,通过PCR技术获得Bt 8010菌株NAD(P)H:醌氧化还原酶基因上下游2个片段,以含有卡那霉素抗性基因的pDG780质粒为中间载体,通过酶切、连接和PCR,构建含待敲除NAD(P)H:醌氧化还原酶基因上下游片段、卡那霉素抗性基因的重组质粒pRN5101DKU,为后续Bt8010菌株NAD(P)H:醌氧化还原酶基因的敲除做准备.
Bacillus thuringiensis subsp.kurstaki isolate 8010 was selected to obtain the upstream and downstream sequences of NAD(P)H: quinone oxidoreductase gene by PCR technology.The pDG780 vector(containing the kanamycin resistance gene) was used to construct a recombination plasmid pRN5101DKU which contained the upstream and downstream sequences of NAD(P)H: quinone oxidoreductase,and the kanamycin resistance gene by PCR and RE treatments.It was provided for further study on gene knockout.
出处
《福建农林大学学报(自然科学版)》
CSCD
北大核心
2012年第5期508-512,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金(31071745)
国家863项目(2011AA10A203)
福建省高校服务海西建设重点项目(0b08b005)