摘要
目的:调查甘肃蒙古族人群无关个体的15个短串联重复序列(STR)基因座(D8S1179,D21S11,D7S820,CSFIP0,D3S1358,D5S818,D13S317,D16S539,D2S1338,D19S433,vWA,D12S391,D18S51,D6S1043,FGA)的遗传多态性。方法:采用AmpFgSTRSinofiler荧光标记复合扩增系统对198名甘肃肃北地区蒙古族个体血样DNA进行15个STR基因座的复合扩增,用3130xl遗传分析仪对扩增产物进行检测。结果:15个STR基因座均符合Hardy-Weinberg平衡,杂合度在0.7071~0.8737之间,累积个人识别概率和累积非父排除率分别为0.9999993和0.999999997。结论:上述15个STR基因座在甘肃蒙古族人群中等位基因分布较好,个体识别率高,适合法医个体识别和亲子鉴定。
Objective To investigate the polymorphism of 15 short tandem repeat (STR) loci (D8Sl179, D21Sll, D7S820, CSF1P0, D3S1358, D5S818, D13S317, D16S539, D2S1338, D19S433, vWA, D12S391, D18S51, D6S1043, FGA) in Mongolian nationality of Gansu province. Methods.. The genotypes of 15 STR loci in Mongolian population of Gansu prov- ince were generated by multiplexing PCR using the Sinofiler PCR system and genotyping using ABI 3130 xl Genetic Analy- zer. Results: All loci met Hardy-Weinberg equilibrium expectations. The heterozygosity of the 15 STR loci was between 0. 707 1 and 0. 873 7, cumulate PE was 0. 999 999 3, and cumulate DP was 0. 999 999 997. Conclusion: The genetic poly- morphisms of the 15 STR loci in Mongolian population of Gansu province are high. Present data suggest that the 15 loci of Sinofiler system can be applied as candidate markers of forensic individual identification and paternity testing.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第5期653-655,共3页
Chinese Journal of Anatomy