食蟹猴妊娠早期血清生殖激素的变化

Detection of serum reproductive hormone in cynomolgus monkey embryo-fetal developmental toxicity evaluation model

目的:通过研究食蟹猴妊娠早期生殖激素的变化,为进行食蟹猴胚胎-胎仔发育毒性试验提供数据支持。方法:食蟹猴在怀孕第0天(gestation day 0,GD0)和怀孕第20天(GD20)采血分离血清检测孕酮水平。怀孕的雌猴于GD27～GD100每周采血1次,采用化学发光法检测血清中雌二醇、孕酮和催乳素的水平。结果:怀孕雌猴GD20的孕酮水平显著高于GD0。GD0～GD27孕酮水平缓慢上升,GD27达到高峰后下降,在GD48至最低,随后至GD100呈逐渐上升趋势。GD0～GD27雌二醇水平也缓慢上升,之后降低,在GD34达到最低水平,随后呈上升趋势,至GD100达到最高。催乳素水平从CD0开始缓慢上升,GD69达最高,后趋于平稳。结论:食蟹猴妊娠早期主要生殖激素随妊娠发展呈现动态变化,孕酮水平可作为早孕诊断的辅助参考。

OBJECTIVE：The study was performed to evaluate change of reproductive hormone level during early pregnancy in cynomolgus monkeys,and to collect background data to support the cynomolgus monkey embryo-fetal developmental toxicity evaluation model.METHODS：Blood samples from cynomolgus monkeys on gestation day 0（CD0） and gestation day 20（GD20） were collected to measure serum progesterone levels.Blood samples from pregnant monkeys were collected once a week during GD27-100 to measure serum estradiol,progesterone and prolactin levels by chemiluminescence method.RESULTS：Progesterone levels of pregnant female monkeys on GD20 were significantly higher than GD0.Progesterone level rose slowly from GD0 to GD27 until a peak,decreased to the trough on GD48,and then gradually rose until GD100.Estradiol level increased slowly from GD0 to GD27 until a peak,decreased to the lowesl level on GD34,and then gradually rose until GD100.Prolactin level began to rise slowly from GD0 to GD69,and remained stable.CONCLUSION：Reproductive hormone levels in cynomolgus monkeys changed dynamically during early pregnancy.Progesterone levels can be used as supportive evidenced of early pregnancy.