栽培药用大黄不同部位中5种蒽醌类衍生物的含量分析

Contents of five kinds of anthraquinones from different medicinal parts of cultivated Rheum officinale

测定栽培药用大黄药材不同部位中游离蒽醌、结合蒽醌及总蒽醌的含量,采用Agela Venusil XBP-C18柱(4.6 mm×250mm,5μm),以甲醇-0.1%磷酸溶液(75∶25)为流动相,流速为1.0 mL/min,检测波长为254 nm,柱温为35℃。结果表明,栽培药用大黄不同部位均含有5种蒽醌类衍生物且含量差异较大,主要分布在根、根茎、须根中,而茎、叶柄、叶片、花序未检出大黄素甲醚。须根、根中游离蒽醌、总蒽醌及结合蒽醌的总含量高于其他部位;地下部分游离蒽醌、总蒽醌及结合蒽醌的总含量显著高于地上部分。该方法能准确简便地提取药用大黄植物中的蒽醌类衍生物并分析其含量,可为合理开发药用大黄提供一定的科学依据。

To determine the content of free anthraquinons, combined anthraquinons and total anthraquinons from different medicinal parts of Rheum officinale, Agela Venusil XBP-CIs （4.6 mm×250 ram,5 p.m） was used with methanol-0.1% posphoric acid solution as mobile phase at the flow rate of 1.0 mL/min, the wavelength was set at 254 nm, temperature was set at 35℃. The results showed that 5 kinds of anthraquinons existed in different medicinal parts of R. officinale and the content was obvious different. The anthraquinons mainly distributed in root, stem and fibrous root, the physcione was not detected in stem, peliole blade and inflorescece. The contents of free anthraquinons, total anthraquinons and combined anthraquinons from fibrous root and root was higher than those of the other, which was much more underground part than that of the aboveground part. Methods established could be easily and exactly used to extract and content determination of anthraquinons in R. officinale and could provide scientific foundation for research and development of R. offic inale .