转基因苜蓿对土壤微生物的影响

Effects of transgenic alfalfa on soil microorganisms

采用传统的平板计数法和PCR凝胶电泳技术,在大田栽培条件下,以转BADH基因苜蓿和非转基因苜蓿为材料,于2009年和2010年连续2年测定苜蓿根际土壤细菌、放线菌和真菌数量的变化,并对外源基因是否转移到土壤微生物中的可能性进行检测分析。结果表明,在不同年份和不同月份转基因植株与非转基因植株根部土壤3大类微生物(细菌、放线菌和真菌)数量变化趋势一致;二者根部土壤3大类微生物数量无显著差异,2年间转基因苜蓿的土壤3大类微生物数量无显著差异;并利用BADH基因特异引物对土样的总DNA,以及菌株DNA进行PCR扩增,均未检测到外源基因扩增产物。初步研究结果表明,转基因苜蓿对土壤微生物系统尚没有明显的影响。

A field release of transgenic alfalfa and non-transgenic alfalfa grown under normal agricultural practice was monitored for changes in total bacterial,actinomycetic and fungal populations in 2009 and 2010.Classical plate counting and PCR technology were adopted to investigate the amounts of microorganisms and horizontal gene transfer.We found that three kinds of soil microorganism appeared with identical trends within different months and different years.ANOVA and multiple comparison analyses revealed that there was no significant difference in terms of the microorganism numbers between both individual plants and between the amounts of cultured soil microorganisms in the rhizosphere soil of the transgenic alfalfa and non-transgenic alfalfa.No alien target DNA was detected from PCR products of total soil DNA,and strains DNA amplified with specific primers of BADH.Our preliminary results indicated that there are still no significant effects of transgenic alfalfa on the soil microorganism system.