中国人2型糖尿病微血管并发症与AGE受体基因Gly82Ser多态性相关研究

A study for RAGE Gly82Ser polymorphism in Chinese type 2 diabetic microangiopathy

目的 探讨 AGE受体 (RAGE)基因 Gly82 Ser多态性与中国人 2型糖尿病微血管并发症间的关系。方法 使用限制性内切酶 Alu I(AG↑ CT)的 PCR- RFL P法 ,检测 10 4例非糖尿病对照者和 15 6例 2型糖尿病伴或不伴肾病、视网膜病变者的 RAGE基因 Gly82 Ser的多态基因型。结果 中国人 RAGE基因 Gly82 Ser多态基因型以 GG型、等位基因以 G型为最多见 ,但频率分布与白种人相比有显著性差异。非 DM对照组与 2型糖尿病伴或不伴有肾病的 4个亚组间、与伴或不伴有视网膜病变的 2个亚组间 ,RAGE基因的 Gly82 Ser多态的基因型 (GG、GS、SS)频率或等位基因 (G、S)频率皆无显著性差异 (Fisher's确切 P值 >0 .0 5 )。结论 RAGE基因的 Gly82 Ser多态引起的 RAGE功能性氨基酸突变与

Objective To investigate the association between a new identified Gly82Ser polymorphism of RAGE gene and diabetic microangiopathy,a case control study of 260 Chinese subjects (including 156 type 2 diabetics with or without nephropathy or retinopathy and 104 non diabetic control) was performed.Methods Genotype frequencies of Gly82Ser polymorphism were studied by PCR RFLP analysis with AluI digestion.Results The most frequent genotype and allele of Gly82Ser polymorphism of RAGE gene in Chinese were genotype GG and allele G.Their frequency distribution showed significant difference between Chinese and Caucasian.There was no difference in genotype frequencies or allele frequencies between type 2 diabetic patients (DN 0) and the control subjects.Genotype and allele frequencies did not differ in type 2 diabetic subjects with nephropathy or without nephropathy.Moreover,there were no associations between diabetic patients with or without retinopathy in genotype and allele frequencies as well (two tailed Fisher's exact P >0.05).Conclusion Our results showed that Gly82Ser polymorphism in RAGE gene was not associated with diabetic microangiopathy,which suggests that these genetic variants may not be involved in the mechanism of diabetic microangiopathy in Chinese type 2 diabetics.