凝胶色谱净化/气相色谱法测定鳗鱼中多种有机磷农药残留量

Determination of Organophosphorus Pesticide Multi-residues in Eel by Gas Chromatography with Gel Permeation Chromatography Clean-up

建立了凝胶色谱技术（GPC）净化，用气相色谱法测定水产品鳗鱼中11种有机磷农药残留量的方法。样品用乙酸乙酯／环己烷溶液（1＋1，V／V）超声提取，GPC净化，收集所需流分，浓缩近干后用乙酸乙酯溶解定容，气相色谱分析。方法检测限（LOQ）为0．01-4）．02mg／kg，方法回收率为76．3％106．8％，相对标准偏差（RSD）为2．3％-15．8％。结果表明，该方法操作简便怏速、灵敏度高，符合实际应用需要。

A method was developed for determining of 11 organophosphorus pesticide residues in eel by Gas Chromatography （GC） with Gel Permeation Chromatography （GPC） clean-up. The samples were extracted using the solution of ethyl acetate and cyclohexane （1＋1, V/V） with ultrasonic machine and then cleaned up by GPC. The elution needed was ccollected and concentrated to nearly dryness. The residue was dissolved with ethyl acetate for determination by GC-FPD. The limit of quantification （LOQ） was 0.01-0.02 mg/kg. The recovery ranges were 76.3%106.8% when the added levels were from 0.02 mg/kg to 1.0 mg/kg. The relative standard deviations （RSD） were 2.6%-13.8%. The method was simple, sensitive and suitable for the analysis of pesticide residues.