太平洋磷虾脂肪酸的气相色谱-质谱法分析

Analysis of Fatty Acids in Euphausia Pacifica by Gas Chromatograph-Mass Spectrometry

通过采用Folch法从太平洋磷虾(Euphausia Pacifica)中提取总脂,通过硅胶柱层析分析了总脂中中性脂、糖脂和磷脂的组成情况,并采用气相色谱-质谱(GC-MS)法对这三类脂质的脂肪酸组成进行了分析和比较。结果表明,太平洋磷虾的总脂含量为15.16%,其中中性脂占总脂含量的70.19%,磷脂占25.15%,糖脂占4.66%。通过气相色谱-质谱分析从太平洋磷虾的总脂中共鉴定出20种脂肪酸,其中不饱和脂肪酸11种,占总脂肪酸总量的55.54%。对中性脂、糖脂和磷脂的脂肪酸组成进行的分析比较表明,不同脂质中脂肪酸组成及其含量存在一定的差异。本文旨在为太平洋磷虾的营养评价和高值化利用提供一定的基础数据。

The total lipids of Euphausia Pacifica were extracted by Folch method, Silica column chromatography method was used to separate total lipids into neutral lipid, glycolipid and phospholipid. The fatty acids were analyzed by Gas Chromatography-Mass Spectrometry （GC-MS） after derivatized. The results showed that the content of total lipid was 15.16%, 70.19% was neutral lipid which was the main lipid, phospholipid was 25.15% while glycolipid was 4.66%. 20 fatty acids were identified in the total lipid extracted from Euphausia Pacifica, including 11 unsaturated fatty acids, the total content of EPA and DHA was 23.36%. According to the comparison and analysis of fatty acids in neutral lipid, glycolipid and phospholipid, the compositions and contents of fatty acids had definite difference. This thesis is intended to provide theory basis for nutritional evaluation and optimum exploitation ofEuphausia Pacifica.