火锅底料中吗啡组分的检测方法研究

Method Research of Detection of Morphine Component in Chafing Dish

采用高效液相色谱法,将组分从固体中转移到液体中是保障定量结果符合真实值的前提,将甲醇溶解法确定为前处理的基本方法。火锅底料中吗啡含量为5.51%、7.60%、10.20%,以C18柱为分析柱,使用国产固相萃取小柱可以得到98.7%的回收率。流动相0.05 mol/L磷酸二氢钾、0.0025 mol/L庚烷磺酸钠水溶液-乙腈;柱温35℃;流速1 mL/min;检测波长220 mm,分析了火锅底料中的吗啡。

Using high performance liquid chromatography, transferring component from solid m liquid is a prerequisite for the protection of quantitative results of real value, water-soluble solution to determine the basic method for pre-treatment. Results showed that the chafing dish morphine content were 5.31%, 7.60%, 10.20% detected by HPLC on a Clscolumn. the use of domestic solid phase extraction column can give recovery rate of 98.7%. The HPLC detection conditions were mobile phase 0.05 mol/L potassium dihydrogen phosphate, 0.0025 mol/L heptane sulfonate aqueous solution - acetonitrile, column temperature 35 ℃, flow rate 1 mL/min, and detection wavelength.