摘要
酶联免疫斑点法(ELISPOT)技术作为一种新的免疫学检测方法用于结核感染的诊断,其主要原理是应用结核分枝杆菌特异性抗原ESAT-6与CFP-10刺激患者外周血T淋巴细胞使T细胞活化释放干扰素γ,根据活化T细胞数来判定是否存在结核感染,此技术因不受机体免疫力及卡介苗接种的影响,而较皮肤结核菌素试验具有更高的特异性及敏感性,现就ELISPOT的技术原理、发展简史、操作步骤及应用进行综述。
Enzyme-linked immunospot（ELISPOT）assay is used in the diagnosis of tuberculosis as a new immunology detection method.The main principle is application of mycobacterium tuberculosis specific antigen ESAT-6,CFP-10 to stimulate peripheral blood T lymphocytes,make T-cell activated and release γ-interferon,to determine whether there are tuberculosis according to activated T-cell number.This technology is with higher sensitivity and specificity than the TST experiment,for it is not affected by body immunity and BCG vaccination,and here is to make a review on ELISPOT technology principle,brief development history,operation procedure and the application.
出处
《医学综述》
2012年第18期3047-3049,共3页
Medical Recapitulate
关键词
酶联免疫斑点法
结核感染
诊断
Enzyme-linked immunospot assay
Ttuberculosis infection
Diagnosis
