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茶树类黄酮3-O-葡萄糖基转移酶基因的克隆和表达分析 被引量：7

The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O. Kuntze]
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摘要 以茶树叶片为材料,结合同源克隆方法和RACE技术,克隆了1条UFGT基因,命名为CsUFGT。该基因cDNA全长为1526bp,ORF长1380bp,编码459个氨基酸,推测等电点5.96,推测分子量为49.486 kDa。该基因编码的蛋白质与葡萄UFGT(P51094.2)的一致性为59%,相似性为75%,其C-端含有植物UGT家族成员特有PSPG基序。荧光实时定量PCR分析表明,该基因在茶树根茎叶中均表达,在第4叶表达量最高,根和茎中表达量较低。 A glucosyltransferase gene UDP-flavonoid 3-O-glucosyl transferase was isolated from tea plant [Camellia sinensis （L.） O. Kuntze] and named CsUFGT. CsUFGT has 1 526 bp full length with open reading frame of 1380 bp which encodes 459 amino acids. The corresponding protein CsUFGT, with predicted molecular mass 49.486 kDa and predicted isoelectric point 5.96, shares 59% identity and 75% similarity with UFGT（PS1094.2） in Vitis vinifer. CsUFGT includes a PSPG signal motif of typical plant glucosyltransferase, qRT-PCR analysis showed that the gene expressed in all tissues of tea plant [Camellia sinensis （L.） O. Kuntze], and had high expression in the fourth leaf and low expression level in root and stem.
作者 王晓帆 田艳维 王云生 高丽萍 夏涛
机构地区 安徽农业大学教育部茶叶生物化学与生物技术重点实验室 安徽农业大学生命科学学院
出处 《茶叶科学》 CAS CSCD 北大核心 2012年第5期411-418,共8页 Journal of Tea Science
基金 国家自然科学基金(30972401 31170647和31170282)
关键词 茶树 类黄酮3-O-葡萄糖基转移酶 基因克隆 表达分析 Camellia sinensis, flavonoid 3-O-glucosyl transferase, gene cloning, expression analysis
分类号 S572 [农业科学—烟草工业]
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参考文献23

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二级参考文献52

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共引文献32

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茶叶科学
2012年 第5期

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