摘要
我们对临床分离的144株金黄色葡萄球菌作了定量耐药谱分型和噬菌体分型。将相似性系数E引人菌株相 似性分析,大大提高了耐药谱分型的辨别力和重复性。我们还采用Hunter介绍的辨别力指数D来评价方法的辨别力, 使辨别力数字化。噬菌体分型除采用国际通用基本分型噬菌体外,还首次将国际中心新建立的一套10个MRSA分型噬 菌体用于我国临床菌株分型。试验表明,定量耐药谱分型的分型率高(100%),重复性好(98.3%),辨别力强 (0.988),且方法简便,是金葡菌流行病学分型的有效方法。而噬菌体分型的分型率较低,为63.2%,有必要继续改进 分型噬菌体。
Staphylococcus aureus isolates were typed by quantitative antibiogram typing and phage typing. We choose the Euclidean distance as a similarity coefficient. To evaluate the discriminatory power of the methods, we calculated an index of discrimination. We performed phage typing with the international basic set and the new set for MRSA. In conclusion, quantitative antibiogram typing, with its high typability (100% ), reproducibility (98. 3% ) and discriminatory power (0. 988), is a powerful method for epidemiological investigation of S. aureus.
出处
《中国卫生检验杂志》
2000年第2期129-131,共3页
Chinese Journal of Health Laboratory Technology
关键词
金葡菌
定量耐药谱分型
噬菌体分型
相似性系数
Staphylococcus aureus, quantitative antibiogram typing, phage typing, similarity coefficient, index of discrimination