CCL21转染PANC-1细胞株及对其生物学行为的影响

Biological Behavior Study of Pancreatic Cancer Cell (PANC-1) Transfected by Lentivirus-mediated hCCL21

目的观察CCL21重组慢病毒载体转染PANC-1细胞株及对其生物学行为的影响。方法构建的CCL21重组慢病毒载体转染胰腺癌细胞株PANC-1,通过PCR、Western blot进行验证。结果构建的重组CCL21-慢病毒成功转染胰腺癌细胞株并稳定表达,但转染后的胰腺癌细胞株PANC-1其生物学行为未发生明显变化。结论通过PCR、Western blot检测,构建的重组CCL21-慢病毒成功转染胰腺癌细胞株并稳定表达,但转染后的胰腺癌细胞株PANC-1其生物学行为未发生明显变化。

Objective In this research, the human CCL21 gene was cloned and pEASY-T1 simple-CCL21 plasmid was constructed. hCCL21 gene was constructed to the lentivirus vector after being sequenced. It was certified that pancreatic cancer cells line PANC-1 expressing CCR7 highly by real-time PCR. The biological behavior of PANC-1 transfected by lentivirus-mediated hCCL21 was observed. Methods MOI was determined by pre-test of lentiviral transfection. PANC-1 cell line was transfected by lentivirus-mediated hCCL21, which was divided into the experimental group, negative control group and blank control group, and it was verified by PCR, Western blot. The biological behavior of PANC-1 cell line transfected by lentivirus-mediated hCCL21 was observed. Results Lenti-hCCL21 successfully transfected pancreatic cancer cell line PANC-1, and stably expressed, however, the biological behavior of PANC-1 cells hade no significant change. Conclusions Lenti-bCCL21 stably transfects pancreatic cancer cell line PANC-1 with high expression of CCR7, but the biological behavior of the positive experimental group has no significant change.