用RAPD技术分析甲型溶血性链球菌DNA

Analyse the DNA and a-hemolytic streptococcus by using RAPD

［目的］应用ＲＡＰＤ技术分析甲型溶血性链球菌ＤＮＡ多态性并对其分型。［方法］随机设计１７ｈｐ和１０ｈｐ 两条引物，采用近年来建立起来的ＲＡＰＤ技术对３０株甲型溶血性链球菌ＤＮＡ进行扩增，扩增产物经凝胶电泳后得到 指纹图。［结果］共出现１２个ＤＮＡ条带，根据指纹图的异同将其分为１、Ｒ、１型别。［结论］甲型溶血性链球 菌 ＤＮＡ用 ＲＡＰＤ技术分析，把该菌进一步分型且方法操作简便，结果准确、稳定。

： [Objective ]: To analyse the DNA polymorphism and identify the strain types in a-hemolytic streptococcus by using Randomly Amplified pdymorphic DNA. [Methods ] Randomly designing 17hp and 10hp oligonucleotide as primers. We used the technique of Randomly Amplified pdymorphic DNA,'which has been developed in recent years, to amplify the DNA of 30 strains of a-hemolytic streptococcus and followed the agarose electrophoresis of the amplified products and the finger prints were revealed. [Result ] Altogether there were 12 scorable markers. The 30 strains were differentiated into I ~ Ⅲ type. [Conclusions ] RAPD is a kind of accurate method to analyze a-hemolytic streptococcus and also a reliable molecular biological method because of the simplified operation and the stable results.