摘要
绿藻素是营养全面的保健食品。ＶＢ_５在绿藻素中的含量丰富。本法以 ０．２５ｍｏｌ／ＬＨ_２ＳＯ_４： ９５％乙醇＝１＋１为 提取剂，流动相为甲醇：异丙醇：十二烷基磺酸钠（０．５ｇ／Ｌ）＝７＋２＋１，并用Ｈ_３ＰＯ_４调节ｐＨ＝２．５，在Ｃ_１８反相柱上 得到了良好的分离。其回收率为９６．０％，相对标准偏差为２．９２％，标准差为０．００５８１，样品提取液经０．４５μｍ滤膜过滤 后可直接上机进样。本法为保健食品绿藻素中的ＶＢ_５的测定提供了可行的检测分析方法。
A method for determination of VBS (nicotinic acid)in health food chlorella by reversed phase high performance liquid chromatography was reported. The VBS was separated on a C18 reversed - phase column using methanol: isopropylalcohol: sodium laurylsulfonate (0. 5g/L) =7: 2: 1 V/V as the mobil phase at a velocity of 1ml/min and detected by ultraviolet spectrophotometric detector at 261nm. Good separation was achieved. The recovery was 96. 0% and the variation coefficient was 2. 92%. After through 0. 45pm filter the extracts of chlorella could be measured directly.
出处
《中国卫生检验杂志》
2000年第2期140-142,共3页
Chinese Journal of Health Laboratory Technology
